Fang Yi, Li Xiaoling
Signal Transduction Laboratory, National Institute of Environmental Health Sciences, Research Triangle Park, Durham, NC 27709, USA.
STAR Protoc. 2021 Jul 7;2(3):100659. doi: 10.1016/j.xpro.2021.100659. eCollection 2021 Sep 17.
Training and experiences are usually required to successfully culture and differentiate human embryonic stem cells (hESCs). Here, we describe a simple but highly efficient protocol to induce endoderm differentiation of hESCs with crotonate, a precursor of crotonyl-CoA for histone crotonylation deposition on endodermal genes. In this protocol, adding crotonate in different endoderm differentiation media significantly increases the differentiation efficiency and substantially reduces the amount of required reagents. For complete details on the use and execution of this protocol, please refer to Fang et al. (2021).
成功培养和分化人类胚胎干细胞（hESCs）通常需要培训和经验。在此，我们描述了一种简单但高效的方案，用巴豆酸盐诱导hESCs向内胚层分化，巴豆酸盐是巴豆酰辅酶A的前体，用于组蛋白巴豆酰化沉积在内胚层基因上。在该方案中，在不同的内胚层分化培养基中添加巴豆酸盐可显著提高分化效率，并大幅减少所需试剂的用量。有关该方案使用和执行的完整详细信息，请参阅Fang等人（2021年）的文章。
