Shin Hyeryun, Kawai Hideki Derek
Department of Bioinformatics, Graduate School of Engineering, Soka University, Hachioji, Tokyo 192-8577, Japan.
Department of Biosciences, Graduate School of Science and Engineering, Soka University, Hachioji, Tokyo 192-8577, Japan.
IBRO Neurosci Rep. 2021 Jun 28;11:29-41. doi: 10.1016/j.ibneur.2021.06.004. eCollection 2021 Dec.
Sensory experience influences proliferation and differentiation of oligodendrocyte progenitor cells (OPCs). Enhanced sensorimotor experience promoted the lineage progression of OPCs and myelination in the gray matter and white matter (WM) of sensorimotor cortex. In the visual cortex, reduced experience reportedly delayed the maturation of myelination in the gray matter, but whether and how such experience alters the subcortical WM is unclear. Here we investigated if binocular enucleation from the onset of eye opening (i.e., P15) affects the cell state of OPCs in mouse primary visual cortex (V1). Proliferative cells in the WM declined nearly half over 3 days from postnatal day (P) 25. A 3-day BrdU-labeling showed gradual decline in proliferation rates from P19 to P28. Binocular enucleation resulted in an increase in the cycling state of the OPCs that were proliferated from P22 to P25 but not before or after this period. This increase in proliferative OPCs was not associated with lineage progression toward differentiated oligodendrocytes. Proliferative OPCs arose mostly due to symmetric cell division but also asymmetric formation of proliferative and quiescent OPCs. By P30, almost all the proliferated cells exited the cell cycle. Maturing oligodendrocytes among the proliferated cells increased at this age, but most of them disappeared over 25 days. The cell density of the maturing oligodendrocytes was unaffected by binocular enucleation, however. These data suggest that binocular enucleation transiently elevates proliferative OPCs in the subcortical WM of V1 during a specific period of the fourth postnatal week without subsequently affecting the number of maturing oligodendrocytes several days later.
感觉经验会影响少突胶质前体细胞（OPC）的增殖和分化。增强的感觉运动经验促进了OPC的谱系进展以及感觉运动皮层灰质和白质（WM）中的髓鞘形成。据报道，在视觉皮层中，经验减少会延迟灰质中髓鞘形成的成熟，但这种经验是否以及如何改变皮层下WM尚不清楚。在这里，我们研究了从睁眼开始（即P15）进行双眼摘除是否会影响小鼠初级视觉皮层（V1）中OPC的细胞状态。WM中的增殖细胞在出生后第（P）25天起的3天内减少了近一半。为期3天的BrdU标记显示，从P19到P28增殖率逐渐下降。双眼摘除导致在P22至P25期间增殖的OPC的循环状态增加，但在此期间之前或之后则没有。增殖性OPC的这种增加与向分化少突胶质细胞的谱系进展无关。增殖性OPC主要是由于对称细胞分裂产生的，但也有增殖性和静止性OPC的不对称形成。到P30时，几乎所有增殖细胞都退出了细胞周期。在这个年龄段，增殖细胞中成熟的少突胶质细胞增加，但其中大多数在25天内消失。然而，成熟少突胶质细胞的细胞密度不受双眼摘除的影响。这些数据表明，双眼摘除在出生后第四周的特定时期内会短暂提高V1皮层下WM中增殖性OPC的数量，而不会在几天后影响成熟少突胶质细胞的数量。
