Universidade Federal de Pernambuco (UFPE), Centro de Ciências da Saúde, Departamento de Ciências Farmacêuticas, Av. Prof. Arthur de Sá, s/n, 50740-525 Recife, PE, Brazil.
Laboratório Farmacêutico do Estado de Pernambuco Governador Miguel Arraes (LAFEPE), Largo de Dois Irmãos, 1117, Dois Irmãos, 52171-010 Recife, PE, Brazil.
An Acad Bras Cienc. 2021 Jul 19;93(2):e20190373. doi: 10.1590/0001-3765202120190373. eCollection 2021.
Syzygium cumini L. Skeels belongs to Myrtaceae family. This species has been recognized by its antidiabetic, anti-inflammatory, and antimicrobial activities. Despite ever-increasing scientific interest for this species there is no pharmacopeia method for characterization and standardization of S. cumini yet. So, toward this aim, the objective of this work was to develop an efficient analytical methodology able to determine polyphenols and tannins content from leaves hydroethanolic extract of S. cumini using Folin-Ciocalteu method by ultraviolet absorption spectrophotometry (UV-Vis). The analytical methodology was developed for the first time in the literature for leaves of this specie shown to be fast and low-cost with results expressed through tannic acid equivalent (TAE). Moreover, the methodology presented selectivity with maximum absorption at 706 nm wavelength, linearity with R2>0.99; limit of detection 0.275 µg TAE mL-1 and 0.102 µg TAE mL-1; limit of quantification 1.046 µg TAE mL-1 and 0.912 µg TAE mL-1 for total polyphenols and total tannins, respectively. Furthermore, the methodology was accurate with recover value greater than 98%, as well as exact, reproductive, and robust with coefficient of variation values less than 15% for both compounds. All the results are found within the fixed limits according to RDC 166/2017.
锡叶藤属隶属于桃金娘科。该物种具有抗糖尿病、抗炎和抗菌活性，因此一直受到越来越多的科学关注。尽管人们对该物种越来越感兴趣，但目前还没有针对锡叶藤的特征描述和标准化的药典方法。因此，本研究的目的是开发一种有效的分析方法，能够使用 Folin-Ciocalteu 法通过紫外吸收分光光度法（UV-Vis）测定锡叶藤叶水醇提取物中的多酚和单宁含量。该分析方法首次在文献中报道了用于该物种的叶片，该方法快速且成本低，结果用单宁酸当量（TAE）表示。此外，该方法具有选择性，在 706nm 波长处具有最大吸收，呈线性关系（R2>0.99）；总多酚和总单宁的检测限分别为 0.275µg TAE mL-1和 0.102µg TAE mL-1；定量限分别为 1.046µg TAE mL-1和 0.912µg TAE mL-1。此外，该方法具有较高的准确度，回收率大于 98%，且精密度、重现性和稳健性良好，两种化合物的变异系数均小于 15%。所有结果均符合 RDC 166/2017 规定的固定限值。
