Watanabe H, Takemoto C, Himori N
Department of Pharmacology, Nippon Roche Research Center, Kamakura, Japan.
Nihon Yakurigaku Zasshi. 1987 Oct;90(4):213-20. doi: 10.1254/fpj.90.213.
We have developed a new method for perfusing isolated and blood-perfused rabbit thoracic aortae by using conscious support rabbits. The vasoconstrictory and vasodilatory response to vasoactive substances were recorded as the decrease or the increase in intra-chamber pressure of the glass container in which the rabbit thoracic aorta was mounted. Examined were the vascular responses to dl-norepinephrine (NE) and l-isoproterenol (Iso) at different pressure loads to the vessel. NE produced a dose-dependent vasoconstriction which was effectively suppressed by i.v. phentolamine (3 mg/kg). In contrast, Iso produced beta-adrenoceptor-mediated vasodilations in a dose-dependent manner (dl-propranolol, 0.5 mg/kg, i.v.). The present experiments were performed at different pressure loads (pressure changes both in the vessel-mounted chamber and a Starling pneumatic resistance set connected in the way of a series circuit). Our newly developed system for perfusion of isolated vascular vessels with conscious support rabbits has some advantages over the existing methods for analysing vascular reactivity in terms of being perfused with arterial blood, having a simulated peripheral resistance apparatus in the circuit and showing a distinct vasodilatory response to i.a. Iso.
我们开发了一种新方法，通过使用清醒的辅助兔来灌注分离的和血液灌注的兔胸主动脉。对血管活性物质的血管收缩和血管舒张反应，记录为安装有兔胸主动脉的玻璃容器腔内压力的降低或升高。研究了血管在不同压力负荷下对去甲肾上腺素（NE）和异丙肾上腺素（Iso）的反应。NE产生剂量依赖性血管收缩，静脉注射酚妥拉明（3mg/kg）可有效抑制。相反，Iso以剂量依赖性方式产生β-肾上腺素能受体介导的血管舒张（静脉注射普萘洛尔，0.5mg/kg）。本实验在不同压力负荷下进行（安装血管的腔室和以串联方式连接的斯塔林气动阻力装置中的压力变化）。我们新开发的用清醒辅助兔灌注分离血管的系统，在分析血管反应性方面比现有方法有一些优势，包括用动脉血灌注、在回路中有模拟外周阻力装置以及对动脉内注射Iso表现出明显的血管舒张反应。
