The Franciszek Górski Institute of Plant Physiology Polish Academy of Sciences, Niezapominajek 21, 30-239, Kraków, Poland.
Department of Plant Genetics, Breeding and Biotechnology, West-Pomeranian University of Technology, Słowackiego 17, 71-434, Szczecin, Poland.
Plant J. 2021 Oct;108(1):93-119. doi: 10.1111/tpj.15428. Epub 2021 Aug 20.
The objective of this research was to investigate the differences between glaucous and non-glaucous near-isogenic lines (NILs) of winter rye (Secale cereale L.) in terms of epicuticular wax layer properties (weight, composition, and crystal morphology), selected physiological and biochemical responses, yield components, above-ground biomass, and plant height under soil drought stress. An important aspect of this analysis was to examine the correlation between the above characteristics. Two different NIL pairs were tested, each consisting of a typical glaucous line and a non-glaucous line with a recessive mutation. The drought experiment was conducted twice (2015-2016). Our study showed that wax accumulation during drought was not correlated with higher leaf hydration and glaucousness. Environmental factors had a large impact on the response of the lines to drought in individual years, both in terms of physiological and biochemical reactions, and the composition of epicuticular leaf wax. The analysed pairs displayed significantly different responses to drought. Demonstration of the correlation between the components of rye leaf wax and the physiological and biochemical parameters of rye NILs is a significant achievement of this work. Interestingly, the study showed a correlation between the wax components and the content of photosynthetic pigments and tocopherols, whose biosynthesis, similarly to the biosynthesis of wax precursors, is mainly located in chloroplasts. This suggests a relationship between wax biosynthesis and plant response to various environmental conditions and drought stress.
本研究旨在探讨冬季黑麦（Secale cereale L.）的有蜡和无蜡近等基因系（NILs）在表皮蜡层特性（重量、组成和晶体形态）、选择的生理生化反应、产量构成、地上生物量和株高方面的差异，以及在土壤干旱胁迫下。分析的一个重要方面是检查上述特征之间的相关性。测试了两个不同的 NIL 对，每个 NIL 对由一个典型的有蜡系和一个具有隐性突变的无蜡系组成。干旱实验进行了两次（2015-2016 年）。我们的研究表明，干旱期间蜡的积累与较高的叶片水合作用和有蜡性无关。环境因素对各年系对干旱的反应有很大影响，无论是在生理生化反应还是表皮叶蜡组成方面。分析的对在对干旱的反应上表现出明显的差异。证明黑麦叶片蜡成分与黑麦 NILs 的生理生化参数之间的相关性是这项工作的一个重要成就。有趣的是，该研究表明蜡成分与光合作用色素和生育酚的含量之间存在相关性，生育酚的生物合成与蜡前体的生物合成类似，主要位于叶绿体中。这表明蜡生物合成与植物对各种环境条件和干旱胁迫的反应之间存在关系。
