Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, 630090 Novosibirsk, Russia.
Vavilov Institute of Plant Genetic Resources (VIR), 190000 Saint Petersburg, Russia.
Int J Mol Sci. 2023 Apr 5;24(7):6762. doi: 10.3390/ijms24076762.
Plant surface properties are crucial determinants of resilience to abiotic and biotic stresses. The outer layer of the plant cuticle consists of chemically diverse epicuticular waxes. The WAX INDUCER1/SHINE subfamily of APETALA2/ETHYLENE RESPONSIVE FACTORS regulates cuticle properties in plants. In this study, four barley genes homologous to the gene were mutated using RNA-guided Cas9 endonuclease. Mutations in one of them, the gene, caused a recessive glossy sheath phenotype associated with β-diketone deficiency. A complementation test for knockout (KO) and mutants showed that and are allelic variants of the same genomic locus. A comparison of the transcriptome from leaf sheaths of KO and wild-type plants revealed a specific and strong downregulation of a large gene cluster residing at the previously known locus. Our findings allowed us to postulate that the WIN1 transcription factor in barley is a master mediator of the β-diketone biosynthesis pathway acting through developmental stage- and organ-specific transactivation of the gene cluster.
植物表面特性是决定其对非生物和生物胁迫抗性的关键因素。植物表皮的外层由化学性质多样的角质层蜡组成。APETALA2/ETHYLENE RESPONSIVE FACTOR 中的 WAX INDUCER1/SHINE 亚家族调节植物的角质层特性。在这项研究中，使用 RNA 引导的 Cas9 内切酶对四个大麦基因进行了同源突变，其中一个基因，即 基因的突变导致了隐性有光泽鞘表型，与β-二酮缺乏有关。对 基因敲除（KO）和 突变体的互补测试表明， 和 是同一基因组位点的等位变异。对 基因敲除和野生型植物叶鞘的转录组比较表明，一个大的基因簇在先前已知的 基因座上特异性且强烈下调。我们的研究结果使我们能够假设，大麦中的 WIN1 转录因子是β-二酮生物合成途径的主要介体，通过发育阶段和器官特异性的 基因簇的转录激活来发挥作用。
