Department of Respiratory and Critical Care Medicine, The Second Xiangya Hospital of Central South University, Changsha, Hunan, China.
Research Unit of Respiratory Disease, Central South University, Changsha, Hunan, China.
PLoS One. 2021 Jul 21;16(7):e0254854. doi: 10.1371/journal.pone.0254854. eCollection 2021.
Non-small cell lung cancer (NSCLC) accounts for 85% of all lung cancers. The drug resistance of NSCLC has clinically increased. This study aimed to screen miRNAs associated with NSCLC using bioinformatics analysis. We hope that the screened miRNA can provide a research direction for the subsequent treatment of NSCLC.
We screened out the common miRNAs after compared the NSCLC-related genes in the TCGA database and GEO database. Selected miRNA was performed ROC analysis, survival analysis, and enrichment analysis (GO term and KEGG pathway).
A total of 21 miRNAs were screened in the two databases. And they were all highly expressed in normal and low in cancerous tissues. Hsa-mir-30a was selected by ROC analysis and survival analysis. Enrichment analysis showed that the function of hsa-mir-30a is mainly related to cell cycle regulation and drug metabolism.
Our study found that hsa-mir-30a was differentially expressed in NSCLC, and it mainly affected NSCLC by regulating the cell cycle and drug metabolism.
非小细胞肺癌（NSCLC）占所有肺癌的 85%。NSCLC 的药物耐药性在临床上有所增加。本研究旨在通过生物信息学分析筛选与 NSCLC 相关的 miRNAs。我们希望筛选出的 miRNA 能为后续 NSCLC 的治疗提供研究方向。
我们在 TCGA 数据库和 GEO 数据库中比较了 NSCLC 相关基因，筛选出常见的 miRNAs。对选定的 miRNA 进行 ROC 分析、生存分析和富集分析（GO 术语和 KEGG 通路）。
从两个数据库中共筛选出 21 个 miRNAs，它们在正常组织中高表达，在癌组织中低表达。通过 ROC 分析和生存分析选择了 hsa-mir-30a。富集分析表明，hsa-mir-30a 的功能主要与细胞周期调控和药物代谢有关。
本研究发现 hsa-mir-30a 在 NSCLC 中差异表达，主要通过调节细胞周期和药物代谢来影响 NSCLC。
