Henan Engineering Research Center of Green Pesticide Creation & Intelligent Pesticide Residue Sensor Detection, Henan Institute of Science and Technology, Xinxiang 453003, China; Sumy National Agrarian University, Sumy 40021, Ukraine.
Henan Engineering Research Center of Green Pesticide Creation & Intelligent Pesticide Residue Sensor Detection, Henan Institute of Science and Technology, Xinxiang 453003, China; Henan Engineering Research Center of Biological Pesticide & Fertilizer Development and Synergistic Application, Henan Institute of Science and Technology, Xinxiang 453003, China.
Food Chem. 2022 Jan 1;366:130563. doi: 10.1016/j.foodchem.2021.130563. Epub 2021 Jul 9.
Herein, a simple, low-cost, environment-friendly strategy was proposed to prepare the composite of three-dimensional hierarchical porous carbon and chitosan, which was applied to modify the glass carbon electrode to fabricate an electrochemical sensor for the determination of niclosamide. The three-dimensional porous carbon with interconnected conductive network, high surface area, and self-generated oxygen-containing functional groups was prepared by salt-templating method with glucose as carbon source and eutectic mixture of LiBr/KBr as both activating and pore-forming agent. During the subsequent ultrasonic process, chitosan with excellent filming property, strong adsorption ability, and good dispersibility was successfully decorated on the obtained porous carbon to further enhance the determination performance of niclosamide. Benefitting from the multi-functional integration of three-dimensional hierarchical porous carbon and chitosan, the fabricated sensor presented a low limit of detection (6.7 nM) in the linear concentration range from 0.01 to 10 µM. Moreover, the fabricated sensor could show good repeatability, reproducibility, stability, and selectivity. Most important, the decent practicability for the detection of niclosamide was obtained in different food samples with low relative standard deviation and satisfactory recoveries. This work provides a very valuable reference for the sensitive determination of niclosamide in food samples.
本文提出了一种简单、低成本、环保的策略，用于制备三维分级多孔碳和壳聚糖复合材料，将其应用于修饰玻碳电极，制备用于测定硝氯酚的电化学传感器。通过以葡萄糖为碳源、LiBr/KBr 共晶混合物为活化和造孔剂的盐模板法制备具有互联导电网络、高比表面积和自生成含氧官能团的三维多孔碳。在随后的超声过程中，成功地将具有优异成膜性能、强吸附能力和良好分散性的壳聚糖修饰在所得的多孔碳上，进一步提高了硝氯酚的测定性能。得益于三维分级多孔碳和壳聚糖的多功能集成，所制备的传感器在 0.01 至 10 μM 的线性浓度范围内表现出低检测限（6.7 nM）。此外，所制备的传感器还表现出良好的重复性、重现性、稳定性和选择性。最重要的是，在不同的食品样品中进行的检测，具有低相对标准偏差和令人满意的回收率，表明该传感器具有良好的实际应用潜力。这项工作为食品样品中硝氯酚的灵敏测定提供了非常有价值的参考。
