College of Forestry, Jiangxi Agricultural University, East China Woody Fragrance and Flavor Engineering Research Center of National Forestry and Grassland Administration, Camphor Engineering Research Center of NFGA/Jiangxi Province, Nanchang 330045, People's Republic of China.
Key Laboratory of Chemical Utilization of Plant Resources of Nanchang, College of Chemistry and Materials, Jiangxi Agricultural University, Nanchang 330045, People's Republic of China.
Nanotechnology. 2022 Aug 15;33(44). doi: 10.1088/1361-6528/ac83c8.
Herein, a facile ratiometric electrochemical method was developed for sensitive sensing of riboflavin (RF) based on hierarchical porous biochar (HPB) modified electrode. In this sensing system, the reference paracetamol (PA) was directly added into electrolyte solution without the requirement of complex immobilization process. HPB derived from KOH-activated Soulangeana sepals displays hierarchical porous structure, high specific surface area and rich oxygen-containing functional groups, which is favorable for RF adsorption and enrichment. Besides, the excellent electronic conductivity and superior electrocatalytic activity of HPB can effectively promote the electrooxidation of RF. Moreover, the dual-signal strategy greatly improves the reproducibility and reliability of electrochemical detection. Based on the proposed ratiometric sensing platform, the sensor exhibits a wider linear range of 0.0007-10M and a lower limit of detection of 0.2 nM. The method also presents good selectivity and has been applied to the determination of RF in milk samples with satisfactory results.
本文开发了一种基于分层多孔生物炭（HPB）修饰电极的简便比率电化学方法，用于灵敏检测核黄素（RF）。在该传感系统中，参考物对乙酰氨基酚（PA）无需复杂的固定化过程，直接添加到电解质溶液中。由 KOH 活化的 Soulangeana 萼片衍生的 HPB 具有分层多孔结构、高比表面积和丰富的含氧官能团，有利于 RF 的吸附和富集。此外，HPB 的优异导电性和卓越的电催化活性可有效促进 RF 的电氧化。此外，双信号策略极大地提高了电化学检测的重现性和可靠性。基于所提出的比率传感平台，该传感器具有更宽的线性范围（0.0007-10M）和更低的检测限（0.2 nM）。该方法还表现出良好的选择性，并已应用于牛奶样品中 RF 的测定，结果令人满意。
