Department of Obstetrics and Gynaecology, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, Shandong, 250021, People's Republic of China.
Department of Surgery, Shandong Rongjun General Hospital, Jinan, Shandong, 250013, People's Republic of China.
Reprod Biol Endocrinol. 2021 Jul 21;19(1):114. doi: 10.1186/s12958-021-00800-6.
The immune mechanism was shown to be involved in the development of adenomyosis. The aim of the current study was to evaluate the expression of the immune checkpoints B7-H2, B7-H3, B7-H4 and PD-L2 in adenomyosis and to explore the effect of mifepristone on the expression of these immune checkpoints.
The expression of B7-H2, B7-H3, B7-H4 and PD-L2 in normal endometria and adenomyosis patient samples treated with or without mifepristone was determined by immunohistochemistry analysis.
In adenomyosis patient samples, the expression of B7-H2, B7-H3 and B7-H4 was increased in the eutopic and ectopic endometria compared with normal endometria, both in the proliferative and secretory phases. Moreover, the expression of B7-H2 and B7-H3 was higher in adenomyotic lesions than in the corresponding eutopic endometria, both in the proliferative and secretory phases. The expression of PD-L2 was higher in adenomyotic lesions than in normal endometria in both the proliferative and secretory phases. In the secretory phase but not the proliferative phase, the expression of B7-H4 and PD-L2 in adenomyotic lesions was significantly higher than that in the corresponding eutopic endometria. In normal endometria and eutopic endometria, the expression of B7-H4 was elevated in the proliferative phase compared with that in the secretory phase, while in the ectopic endometria, B7-H4 expression was decreased in the proliferative phase compared with the secretory phase. In addition, the expression of B7-H2, B7-H3, B7-H4 and PD-L2 was significantly decreased in adenomyosis tissues after treatment with mifepristone.
The expression of the immune checkpoint proteins B7-H2, B7-H3, B7-H4 and PD-L2 is upregulated in adenomyosis tissues and is downregulated with mifepristone treatment. The data suggest that B7 immunomodulatory molecules are involved in the pathophysiology of adenomyosis.
免疫机制被认为参与了子宫腺肌病的发生发展。本研究旨在评估免疫检查点 B7-H2、B7-H3、B7-H4 和 PD-L2 在子宫腺肌病中的表达，并探讨米非司酮对这些免疫检查点表达的影响。
通过免疫组织化学分析检测正常子宫内膜和接受或未接受米非司酮治疗的子宫腺肌病患者样本中 B7-H2、B7-H3、B7-H4 和 PD-L2 的表达。
在子宫腺肌病患者样本中，与正常子宫内膜相比，B7-H2、B7-H3 和 B7-H4 在增生期和分泌期的在位和异位子宫内膜中表达增加。此外，在增生期和分泌期，B7-H2 和 B7-H3 在子宫腺肌病病灶中的表达均高于相应的在位子宫内膜。在增生期和分泌期，PD-L2 在子宫腺肌病病灶中的表达均高于正常子宫内膜。在分泌期而非增生期，子宫腺肌病病灶中 B7-H4 和 PD-L2 的表达明显高于相应的在位子宫内膜。在正常子宫内膜和在位子宫内膜中，B7-H4 的表达在增生期高于分泌期，而在异位子宫内膜中，B7-H4 的表达在增生期低于分泌期。此外，米非司酮治疗后子宫腺肌病组织中 B7-H2、B7-H3、B7-H4 和 PD-L2 的表达显著降低。
免疫检查点蛋白 B7-H2、B7-H3、B7-H4 和 PD-L2 在子宫腺肌病组织中的表达上调，并在米非司酮治疗后下调。这些数据表明 B7 免疫调节分子参与了子宫腺肌病的病理生理过程。
