Li Xianchang, Li Chengbo, Zhang Shiding, Cui Chaojun, Li Jianxin, Gao Qianqian
Henan Joint International Research Laboratory of Nanocomposite Sensing Materials, Anyang Institute of Technology, Anyang, 455000, China.
Anal Bioanal Chem. 2021 Sep;413(23):5725-5731. doi: 10.1007/s00216-021-03547-6. Epub 2021 Jul 21.
Developing ultrasensitive and user-friendly methods for the detection glucose has attracted more and more attention. By virtue of high selectivity and sensitivity, enzyme-based glucose sensor plays a key role in point-of-care sensing technology for detecting glucose concentration. In this study, Amplex Red (AR), as both indicator and mediator, was investigated to detect glucose in presence of glucose oxidase (GOx) enzymes using colorimetric and electrochemical methods. Without using any advanced techniques and sophisticated nanomaterials, 1 μM glucose can be easily detected through simply detecting the solution color with a visual colorimetric method. On the other hand, the electrochemical method can provide much higher sensitivity for the detection of glucose, which achieves a linear range spanning from 20 nM to 3.56 μM with a limit of 7.3 nM (signal-to-noise ratio SNR = 3). It is also found that the presence of other sugars such as fructose, lactose, and maltose have very limited interference effects on the detection of glucose. More importantly, a bare GC electrode was used in all these electrochemical measurements without any electrode surface modification, guaranteeing a simple and fast operation. The analytical platforms for the detection of glucose presented here not only provide simple, fast, and ultrasensitive methods, but also have the potential to advance the sensing technology in the application of other health diagnostic research areas. Amplex Red (AR) was reported as both an indicator and mediator for the sensitive and specific determination of glucose using the colorimetric and electrochemical methods. The detection limit was 1 μM glucose by the visual colorimetric methods. A bare glassy carbon electrode without any functional modification was employed for the detection as low as 20 nM glucose with LOD of 7.3 nm (SNR = 3) in the electrochemical method.
开发用于检测葡萄糖的超灵敏且用户友好的方法已引起越来越多的关注。基于酶的葡萄糖传感器凭借其高选择性和灵敏度，在用于检测葡萄糖浓度的即时检测技术中发挥着关键作用。在本研究中，利用比色法和电化学方法，对作为指示剂和介质的Amplex Red（AR）在葡萄糖氧化酶（GOx）存在的情况下检测葡萄糖进行了研究。无需使用任何先进技术和复杂的纳米材料，通过简单的目视比色法检测溶液颜色，就能轻松检测出1μM的葡萄糖。另一方面，电化学方法对葡萄糖检测具有更高的灵敏度，其线性范围为20 nM至3.56μM，检测限为7.3 nM（信噪比SNR = 3）。还发现，果糖、乳糖和麦芽糖等其他糖类的存在对葡萄糖检测的干扰作用非常有限。更重要的是，在所有这些电化学测量中均使用了未经过任何电极表面修饰的裸玻碳电极，保证了操作简单快速。本文介绍的用于检测葡萄糖的分析平台不仅提供了简单、快速和超灵敏的方法，而且在其他健康诊断研究领域的应用中具有推动传感技术发展的潜力。据报道，Amplex Red（AR）作为指示剂和介质，可使用比色法和电化学方法灵敏且特异地测定葡萄糖。目视比色法检测葡萄糖的检测限为1μM。在电化学方法中，使用未经过任何功能修饰的裸玻碳电极检测低至20 nM的葡萄糖，检测限为7.3 nm（SNR = 3）。
