Department of Chemistry, Erzincan Binali Yıldırım University, Erzincan, 24100, Turkey.
Department of Chemistry, Erzincan Binali Yıldırım University, Erzincan, 24100, Turkey.
Colloids Surf B Biointerfaces. 2021 Oct;206:111977. doi: 10.1016/j.colsurfb.2021.111977. Epub 2021 Jul 15.
Graphene oxide (GO), one of the popular materials in recent years, has been synthesized according to the modified Hummers' method. Stable dispersions of different amounts of negatively charged GO were prepared in aqueous media. The GO/dye composites were prepared in deionized water by a simple method with the positively charged Safranin T compound (SfT), which is known to have strong fluorescence properties. By changing the GO/dye ratio, it was obtained stable composites in aqueous media. It was investigated the interaction of SfT with GO in an aqueous solution under the critical micelle concentration (CMC), at the CMC and above the CMC by adding sodium dodecyl sulfate (SDS). It was clarified the formation of GO-SfT composites by using spectroscopic techniques. In these composites, the effect of GO layers on the SfT's photophysical properties was examined by absorption and fluorescence spectroscopy techniques. The change in the quenching effect of GO was monitored both adding a stabilizing electrolyte (NaCl) to the media and changing the pH of the medium. The evaluation was on the probability of the GO-SfT-SDS ternary system being a pH sensing biological sensor material. As a result of this study, it was thought that the GO-SfT-SDS system could be functionalized as a fluorescent pH sensor by taking advantage of GO's sensitivity to pH.
氧化石墨烯（GO）是近年来流行的材料之一，根据改良的 Hummers 法进行合成。在水介质中制备了不同电荷量的稳定 GO 分散体。通过简单的方法，将带正电荷的藏红 T 化合物（SfT）与 GO 在去离子水中制备 GO/染料复合材料，已知 SfT 具有很强的荧光性质。通过改变 GO/染料的比例，可以在水介质中获得稳定的复合材料。在临界胶束浓度（CMC）下、CMC 以上和添加十二烷基硫酸钠（SDS）时，研究了 SfT 在水溶液中与 GO 的相互作用。通过光谱技术阐明了 GO-SfT 复合材料的形成。在这些复合材料中，通过吸收和荧光光谱技术研究了 GO 层对 SfT 光物理性质的影响。通过向介质中添加稳定电解质（NaCl）和改变介质的 pH 值来监测 GO 猝灭效应的变化。评估了 GO-SfT-SDS 三元体系作为 pH 感应生物传感器材料的可能性。通过这项研究，认为可以利用 GO 对 pH 值的敏感性，将 GO-SfT-SDS 体系功能化为荧光 pH 传感器。
