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探索生物相互作用：一种新型吡唑啉作为用于能量转移和细胞染色应用的多功能荧光探针。

Exploring Biological Interactions: A New Pyrazoline as a Versatile Fluorescent Probe for Energy Transfer and Cell Staining Applications.

机构信息

Faculty of Arts and Sciences, Department of Chemistry, Erzincan Binali Yıldırım University, Erzincan, Türkiye.

出版信息

ChemistryOpen. 2023 Sep;12(9):e202300092. doi: 10.1002/open.202300092.

DOI:10.1002/open.202300092
PMID:37667461
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10477408/
Abstract

Fluorescent dyes are used in biological systems, because they are highly sensitive and selective. In this work, we investigated the fluorescent probe properties of 2-(5-(pyridin-2-yl)-1H-pyrazol-3-yl) phenol (PYDP) in two media [sodium dodecyl sulfate (SDS) and human serum albumin (HSA)]. Energy transfer parameters, photophysical and thermodynamic parameters of probe were determined. We investigated cytotoxicity of PYDP against colorectal adenocarcinoma cell lines (HT-29), breast cancer cell lines (MCF-7) and 3T3-L1 adipocytes (3T3 L1) cells. The cell staining property of PYDP was monitored using a confocal microscope. The results showed that PYDP binds to HSA, bindings are due to electrostatic/ionic interactions, and the binding process is spontaneous. PYDP was found to exhibit negligible cytotoxicity at high concentrations, and confocal microscope images showed that PYDP stained the cytoplasm of MCF-7 cells.

摘要

荧光染料在生物系统中被广泛应用，因为它们具有高灵敏度和高选择性。在这项工作中，我们研究了 2-(5-(吡啶-2-基)-1H-吡唑-3-基)苯酚(PYDP)在两种介质[十二烷基硫酸钠(SDS)和人血清白蛋白(HSA)]中的荧光探针性质。测定了探针的能量转移参数、光物理和热力学参数。我们研究了 PYDP 对结直肠腺癌细胞系(HT-29)、乳腺癌细胞系(MCF-7)和 3T3-L1 脂肪细胞(3T3L1)的细胞毒性。使用共聚焦显微镜监测了 PYDP 的细胞染色性质。结果表明，PYDP 与 HSA 结合，结合是由于静电/离子相互作用，且结合过程是自发的。PYDP 在高浓度时表现出可忽略的细胞毒性，共聚焦显微镜图像显示 PYDP 染色了 MCF-7 细胞的细胞质。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a90/10477408/667a816a1206/OPEN-12-e202300092-g008.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a90/10477408/6958010a5c48/OPEN-12-e202300092-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a90/10477408/667a816a1206/OPEN-12-e202300092-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a90/10477408/d2292f29699e/OPEN-12-e202300092-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a90/10477408/d1e3736a4e8c/OPEN-12-e202300092-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a90/10477408/1974003e644f/OPEN-12-e202300092-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a90/10477408/f62a8caff9ed/OPEN-12-e202300092-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a90/10477408/f7febc9f6e6e/OPEN-12-e202300092-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a90/10477408/13ea5d0fab6c/OPEN-12-e202300092-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a90/10477408/bfbf182e4711/OPEN-12-e202300092-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a90/10477408/adc6018acdbc/OPEN-12-e202300092-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a90/10477408/74572f5ff998/OPEN-12-e202300092-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a90/10477408/6958010a5c48/OPEN-12-e202300092-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5a90/10477408/667a816a1206/OPEN-12-e202300092-g008.jpg

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