Shimoda N, Muramatsu H, Osawa T, Yamada T, Adachi M, Muramatsu T
Department of Biochemistry, Faculty of Medicine, Kagoshima University.
J Biochem. 1987 Sep;102(3):657-64. doi: 10.1093/oxfordjournals.jbchem.a122101.
Receptors for peanut agglutinin (PNA) were isolated from Kato III human gastric cancer cells by affinity chromatography on PNA agarose, and were labeled by the galactose oxidase-NaB3H4 method. Alkaline NaBH4 treatment of the labeled receptors released two small oligosaccharide alcohols, which were identified as Gal beta 1----3GalNAc-ol and Gal beta 1----4GlcNAc beta 1----6(Gal beta 1----3)GalNAc-ol. Higher oligosaccharides and glycopeptides of both N- and O-linked type were also detected, but they did not appear to bear PNA binding sites. The presence of oligo-N-acetyllactosamine units in the N-linked type sugars was indicated by endo-beta-galactosidase digestion.
通过在花生凝集素（PNA）琼脂糖上进行亲和层析，从人胃癌Kato III细胞中分离出PNA受体，并用半乳糖氧化酶-NaB3H4法进行标记。用碱性NaBH4处理标记的受体后，释放出两种小的低聚糖醇，鉴定为Galβ1----3GalNAc-ol和Galβ1----4GlcNAcβ1----6（Galβ1----3）GalNAc-ol。还检测到了较高的N-连接型和O-连接型低聚糖及糖肽，但它们似乎不带有PNA结合位点。内切β-半乳糖苷酶消化表明N-连接型糖中存在低聚N-乙酰乳糖胺单元。
