Bao Li-Qiong, Chen Tong, Jin Bao-Long, Li Feng-Sheng, Li Zuo-Jun, Chen Mei-Lan, Wang Tie-Lin, Cui Guang-Hong, Huang Lu-Qi
School of Pharmacy,Nanjing University of Chinese Medicine Nanjing 210023,China State Key Laboratory Breeding Base of Dao-di Herbs,National Resource Center for Chinese Meteria Medica,China Academy of Chinese Medical Sciences Beijing 100700,China.
State Key Laboratory Breeding Base of Dao-di Herbs,National Resource Center for Chinese Meteria Medica,China Academy of Chinese Medical Sciences Beijing 100700,China.
Zhongguo Zhong Yao Za Zhi. 2021 Jun;46(11):2806-2815. doi: 10.19540/j.cnki.cjcmm.20210228.101.
The plant root-associated microbiomes include root microbiome and rhizosphere microbiome, which are closely related to plant life activities. Nearly 30% of photosynthesis products of plants are used to synthesize root compounds, there is evidence that root compounds regulate and significantly affect the root microbiome Tanshinones are the main hydrophobic components in Salvia miltiorrhiza. In order to study whether these compounds can regulate the root-associated microbiomes of S. miltiorrhiza, our study first identified a white root S. miltiorrhiza(BG) which contains little tanshinones. Retain of the fifth intron of tanshinones synthesis key enzyme gene SmCPS1 leading to the early termination of the SmCPS1 gene, and a stable white root phenotype. Further, wild type(WT) and BG were planted in greenhouse with nutrient soil(Pindstrup, Denmark) and Shandong soil(collected from the S. miltiorrhiza base in Weifang, Shandong), then high-throughput sequencing was used to analyze the root-associated microbiomes. The results showed that the tanshinones significantly affected the root-associated microbiomes of S. miltiorrhiza, and the impact on root microbiomes was more significant. There are significant differences between WT and BG root microbiomes in species richness, dominant strains and co-occurrence network. Tanshinones have a certain repelling effect on Bacilli which belongs to Gram-positive, while specifically attract some Gram-negative bacteria such as Betaproteobacteria and some specific genus of Alphaproteobacteria. This study determined the important role of tanshinones in regulating the structure of root-associated microbiomes from multiple angles, and shed a light for further improving the quality and yield of S. miltiorrhiza through microenvironment regulation.
植物根际微生物群落包括根微生物群落和根际微生物群落，它们与植物生命活动密切相关。植物近30%的光合作用产物用于合成根系化合物，有证据表明根系化合物可调节并显著影响根际微生物群落。丹参酮是丹参中的主要疏水性成分。为了研究这些化合物是否能调节丹参的根际微生物群落，我们的研究首先鉴定出一种丹参酮含量极少的白根丹参（BG）。丹参酮合成关键酶基因SmCPS1的第五个内含子保留导致SmCPS1基因提前终止，并呈现稳定的白根表型。进一步地，将野生型（WT）和BG种植在装有丹麦品氏托普营养土和山东土壤（从山东潍坊丹参种植基地采集）的温室中，然后利用高通量测序分析根际微生物群落。结果表明，丹参酮显著影响丹参的根际微生物群落，对根微生物群落的影响更为显著。WT和BG根微生物群落在物种丰富度、优势菌株和共现网络方面存在显著差异。丹参酮对革兰氏阳性菌芽孢杆菌有一定的排斥作用，而特异性吸引一些革兰氏阴性菌，如β-变形菌和一些特定属的α-变形菌。本研究从多个角度确定了丹参酮在调节根际微生物群落结构中的重要作用，为通过微环境调控进一步提高丹参的品质和产量提供了思路。
