• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

计算机预测吡丙醚和 4'-OH-吡丙醚对 和 受体作用机制。

In Silico Prediction of the Mechanism of Action of Pyriproxyfen and 4'-OH-Pyriproxyfen against and Receptors.

机构信息

Molecular Modeling Lab, Department of Food and Drug, University of Parma, Parco Area Delle Scienze 17/A, I-43124 Parma, Italy.

出版信息

Int J Mol Sci. 2021 Jul 20;22(14):7751. doi: 10.3390/ijms22147751.

DOI:10.3390/ijms22147751
PMID:34299368
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8306554/
Abstract

BACKGROUND

Poisoning from pesticides can be extremely hazardous for non-invasive species, such as bees, and humans causing nearly 300,000 deaths worldwide every year. Several pesticides are recognized as endocrine disruptors compounds that alter the production of the normal hormones mainly by acting through their interaction with nuclear receptors (NRs). Among the insecticides, one of the most used is pyriproxyfen. As analogous to the juvenile hormone, the pyriproxyfen acts in the bee's larval growth and creates malformations at the adult organism level.

METHODS

This work aims to investigate the possible negative effects of pyriproxyfen and its metabolite, the 4'-OH-pyriproxyfen, on human and bee health. We particularly investigated the mechanism of binding of pyriproxyfen and its metabolite with ultraspiracle protein/ecdysone receptor (USP-EcR) dimer of and the relative heterodimer farnesoid X receptor/retinoid X receptor alpha (FXR-RXRα) of using molecular dynamic simulations.

RESULTS

The results revealed that pyriproxyfen and its metabolite, the 4'-OH- pyriproxyfen, stabilize each dimer and resulted in stronger binders than the natural ligands.

CONCLUSION

We demonstrated the endocrine interference of two pesticides and explained their possible mechanism of action. Furthermore, in vitro studies should be carried out to evaluate the biological effects of pyriproxyfen and its metabolite.

摘要

背景

对于非侵入性物种，如蜜蜂，农药中毒可能会极其危险，每年在全球范围内造成近 30 万人死亡。几种农药被认为是内分泌干扰化合物，它们通过与核受体（NR）的相互作用来改变正常激素的产生。在杀虫剂中，使用最广泛的之一是吡虫啉。吡虫啉类似于保幼激素，作用于蜜蜂幼虫的生长，在成虫水平上造成畸形。

方法

本研究旨在探讨吡虫啉及其代谢物 4'-OH-吡虫啉对人类和蜜蜂健康的可能负面影响。我们特别研究了吡虫啉及其代谢物与 ultraspiracle 蛋白/蜕皮激素受体（USP-EcR）二聚体（和相对的法尼醇 X 受体/视黄酸 X 受体α（FXR-RXRα）二聚体的结合机制。使用分子动力学模拟。

结果

结果表明，吡虫啉及其代谢物 4'-OH-吡虫啉稳定了每个二聚体，并成为比天然配体更强的结合物。

结论

我们证明了两种农药的内分泌干扰，并解释了它们可能的作用机制。此外，应进行体外研究以评估吡虫啉及其代谢物的生物学效应。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7e10/8306554/155eee697d7b/ijms-22-07751-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7e10/8306554/cc184b9d6952/ijms-22-07751-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7e10/8306554/09fec94feaee/ijms-22-07751-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7e10/8306554/535004c489e6/ijms-22-07751-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7e10/8306554/efb68e0a17ae/ijms-22-07751-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7e10/8306554/6d6a8a89f0a0/ijms-22-07751-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7e10/8306554/37f22d4e1ca9/ijms-22-07751-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7e10/8306554/c6cf33327446/ijms-22-07751-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7e10/8306554/155eee697d7b/ijms-22-07751-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7e10/8306554/cc184b9d6952/ijms-22-07751-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7e10/8306554/09fec94feaee/ijms-22-07751-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7e10/8306554/535004c489e6/ijms-22-07751-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7e10/8306554/efb68e0a17ae/ijms-22-07751-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7e10/8306554/6d6a8a89f0a0/ijms-22-07751-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7e10/8306554/37f22d4e1ca9/ijms-22-07751-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7e10/8306554/c6cf33327446/ijms-22-07751-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7e10/8306554/155eee697d7b/ijms-22-07751-g008.jpg

相似文献

1
In Silico Prediction of the Mechanism of Action of Pyriproxyfen and 4'-OH-Pyriproxyfen against and Receptors.计算机预测吡丙醚和 4'-OH-吡丙醚对 和 受体作用机制。
Int J Mol Sci. 2021 Jul 20;22(14):7751. doi: 10.3390/ijms22147751.
2
Larval Exposure to the Juvenile Hormone Analog Pyriproxyfen Disrupts Acceptance of and Social Behavior Performance in Adult Honeybees.幼虫接触保幼激素类似物吡丙醚会破坏成年蜜蜂的接受能力和社会行为表现。
PLoS One. 2015 Jul 14;10(7):e0132985. doi: 10.1371/journal.pone.0132985. eCollection 2015.
3
Influence of the insecticide pyriproxyfen on the flight muscle differentiation of Apis mellifera (Hymenoptera, Apidae).杀虫剂吡丙醚对蜜蜂（膜翅目，蜜蜂科）飞行肌分化的影响。
Microsc Res Tech. 2012 Jun;75(6):844-8. doi: 10.1002/jemt.22003. Epub 2012 Jan 6.
4
Effects of Pyriproxyfen on Female Reproduction in the Common Cutworm, Spodoptera litura (F.) (Lepidoptera: Noctuidae).吡丙醚对斜纹夜蛾（Spodoptera litura (F.)）（鳞翅目：夜蛾科）雌性生殖的影响
PLoS One. 2015 Oct 7;10(10):e0138171. doi: 10.1371/journal.pone.0138171. eCollection 2015.
5
The Effects of the Insect Growth Regulators Methoxyfenozide and Pyriproxyfen and the Acaricide Bifenazate on Honey Bee (Hymenoptera: Apidae) Forager Survival.昆虫生长调节剂甲氧虫酰肼和吡丙醚以及杀螨剂联苯肼酯对蜜蜂（膜翅目：蜜蜂科）觅食者存活率的影响
J Econ Entomol. 2018 Apr 2;111(2):510-516. doi: 10.1093/jee/tox347.
6
Interactions of ultraspiracle with ecdysone receptor in the transduction of ecdysone- and juvenile hormone-signaling.在蜕皮激素和保幼激素信号转导过程中超气门蛋白与蜕皮激素受体的相互作用。
FEBS J. 2005 Apr;272(7):1577-89. doi: 10.1111/j.1742-4658.2005.04578.x.
7
Expression, subcellular localization and protein-protein interaction of four isoforms of EcR/USP in the common cutworm.蜕皮激素受体/泛素特异性蛋白酶在斜纹夜蛾四个同工型的表达、亚细胞定位和蛋白-蛋白相互作用
Insect Sci. 2015 Feb;22(1):95-105. doi: 10.1111/1744-7917.12101. Epub 2014 Mar 19.
8
The inhibition of ecdysone signal pathway was the key of pyriproxyfen poisoning for silkworm, Bombyx mori.抑制蜕皮激素信号通路是吡丙醚对家蚕中毒的关键。
Pestic Biochem Physiol. 2023 Jan;189:105307. doi: 10.1016/j.pestbp.2022.105307. Epub 2022 Dec 1.
9
Lethal and Sublethal Effects of Pyriproxyfen on and Non- Bees.吡丙醚对蜜蜂和非蜜蜂的致死及亚致死效应。
Toxics. 2020 Nov 17;8(4):104. doi: 10.3390/toxics8040104.
10
Quantitative determination of pyriproxyfen and its metabolite residues in bee products of China using a modified QuEChERS approach with UPLC-MS/MS.采用改良的 QuEChERS 法结合 UPLC-MS/MS 测定中国蜂蜜中的吡丙醚及其代谢物残留量。
Ecotoxicol Environ Saf. 2021 Sep 1;220:112388. doi: 10.1016/j.ecoenv.2021.112388. Epub 2021 Jun 3.

本文引用的文献

1
Lethal and Sublethal Effects of Pyriproxyfen on and Non- Bees.吡丙醚对蜜蜂和非蜜蜂的致死及亚致死效应。
Toxics. 2020 Nov 17;8(4):104. doi: 10.3390/toxics8040104.
2
Factors Associated with Honey Bee Colony Losses: A Mini-Review.与蜜蜂蜂群损失相关的因素：一篇综述短文
Vet Sci. 2020 Oct 30;7(4):166. doi: 10.3390/vetsci7040166.
3
Scalable molecular dynamics on CPU and GPU architectures with NAMD.使用 NAMD 在 CPU 和 GPU 架构上进行可扩展的分子动力学。
J Chem Phys. 2020 Jul 28;153(4):044130. doi: 10.1063/5.0014475.
4
Peer review of the pesticide risk assessment of the active substance pyriproxyfen.活性物质吡丙醚的农药风险评估同行评审
EFSA J. 2019 Jul 17;17(7):e05732. doi: 10.2903/j.efsa.2019.5732. eCollection 2019 Jul.
5
Why bees are critical for achieving sustainable development.为什么蜜蜂对实现可持续发展至关重要。
Ambio. 2021 Jan;50(1):49-59. doi: 10.1007/s13280-020-01333-9. Epub 2020 Apr 20.
6
Toxicity risk assessment of pyriproxyfen and metabolites in the rat liver: A vitro study.吡丙醚及其代谢物在大鼠肝脏中的毒性风险评估：一项体外研究。
J Hazard Mater. 2020 May 5;389:121835. doi: 10.1016/j.jhazmat.2019.121835. Epub 2019 Dec 5.
7
Pesticides in the urban environment: A potential threat that knocks at the door.城市环境中的农药：敲响家门的潜在威胁。
Sci Total Environ. 2020 Apr 1;711:134612. doi: 10.1016/j.scitotenv.2019.134612. Epub 2019 Nov 14.
8
The Evolution of Insect Metamorphosis.昆虫变态的演化。
Curr Biol. 2019 Dec 2;29(23):R1252-R1268. doi: 10.1016/j.cub.2019.10.009.
9
Persistence of pesticides-based contaminants in the environment and their effective degradation using laccase-assisted biocatalytic systems.利用漆酶辅助生物催化系统在环境中持久性农药类污染物的有效降解。
Sci Total Environ. 2019 Dec 10;695:133896. doi: 10.1016/j.scitotenv.2019.133896. Epub 2019 Aug 13.
10
Pyriproxyfen, a juvenile hormone analog, damages midgut cells and interferes with behaviors of larvae.吡丙醚，一种保幼激素类似物，会损害中肠细胞并干扰幼虫的行为。
PeerJ. 2019 Sep 4;7:e7489. doi: 10.7717/peerj.7489. eCollection 2019.