Oosaki T, Sekiya S, Takamizawa H
Department of Obstetrics and Gynecology, Chiba University School of Medicine.
Nihon Sanka Fujinka Gakkai Zasshi. 1987 Dec;39(12):2137-43.
We have studied biologically active substances, tumor angiogenesis factors (TAF), which are supposed to be associated with the proliferation and metastases of choriocarcinoma cells. Eight human choriocarcinoma cell lines were used in the present study. TAF activity was assayed by bioassays using BALB/c mice subcutaneous tissue, chicken chorioallantoic membrane (CAM) and rabbit cornea in vivo and by proliferation of endothelial cells in vitro. 1. We found a positive correlation between the size of tumors developed in xenotransplantation and the number of blood vessels in the tumor tissues. A correlation between the number of blood vessels and TAF activity was also found. 2. With a gel-filtration method, TAF activity was observed in the common fractions in every cell line. The molecular weight of TAF was more than 10,000 daltons. 3. There was a heterogeneity of TAF activity among the cell lines. From these results, it appears that TAF initiates the choriocarcinoma cell-proliferation.
我们研究了生物活性物质，即肿瘤血管生成因子（TAF），其被认为与绒毛膜癌细胞的增殖和转移有关。本研究使用了8种人绒毛膜癌细胞系。通过使用BALB/c小鼠皮下组织、鸡绒毛尿囊膜（CAM）和兔角膜进行体内生物测定以及通过体外内皮细胞增殖来测定TAF活性。1. 我们发现异种移植中形成的肿瘤大小与肿瘤组织中的血管数量之间存在正相关。还发现了血管数量与TAF活性之间的相关性。2. 使用凝胶过滤法，在每个细胞系的共同组分中观察到TAF活性。TAF的分子量超过10,000道尔顿。3. 细胞系之间存在TAF活性的异质性。从这些结果来看，似乎TAF启动了绒毛膜癌细胞的增殖。
