Sekiya S, Oosaki T, Takamizawa H
Gynecol Oncol. 1986 Nov;25(3):271-80. doi: 10.1016/0090-8258(86)90078-8.
The growth of five different kinds of human choriocarcinoma cell lines in vitro was quite alike. However, two distinct types of rapid and slow growth were observed in tumors grown in the hamster cheek pouch. The rapidly grown tumor tissues (BeWo, JEG-3, and NUC-1) involved significantly large numbers of blood vessels per microscopic field, as compared to slowly grown ones (SCH and HM). The vascular response was assayed using cell-free crude tumor angiogenesis factors (TAF) on chorioallantoic membrane (CAM) of the chick embryo. In all cell lines TAF activity correlated well with the extent of tumor growth in vivo. Gel filtration analysis showed that relatively high-molecular-weight (more than 10,000) factors could induce neovascularization in the both assays using CAM and rabbit cornea. These results suggest that a heterogeneity of TAF activity is present among human choriocarcinoma cell lines and tumor growth in xenograft depends on the secretion of TAF by the tumor cells themselves.
五种不同类型的人绒毛膜癌细胞系在体外的生长情况颇为相似。然而，在仓鼠颊囊内生长的肿瘤中观察到了两种明显不同的快速生长和缓慢生长类型。与生长缓慢的肿瘤组织（SCH和HM）相比，快速生长的肿瘤组织（BeWo、JEG - 3和NUC - 1）在每个显微镜视野中涉及的血管数量显著更多。使用无细胞粗制肿瘤血管生成因子（TAF）在鸡胚绒毛尿囊膜（CAM）上检测血管反应。在所有细胞系中，TAF活性与体内肿瘤生长程度密切相关。凝胶过滤分析表明，相对高分子量（超过10,000）的因子在使用CAM和兔角膜的两种检测中均可诱导新血管形成。这些结果表明，人绒毛膜癌细胞系中存在TAF活性的异质性，异种移植中的肿瘤生长取决于肿瘤细胞自身分泌TAF。
