人心肌细胞亚型的鉴定。

Characterization of Ventricular and Atrial Cardiomyocyte Subtypes from Human-Induced Pluripotent Stem Cells.

机构信息

Department of Cell Growth and Differentiation, Center for iPS Cell Research and Application, Kyoto University, Kyoto, Japan.

Takeda-CiRA Joint Program (T-CiRA), Fujisawa, Kanagawa, Japan.

出版信息

Methods Mol Biol. 2021;2320:135-149. doi: 10.1007/978-1-0716-1484-6_14.

DOI:10.1007/978-1-0716-1484-6_14

PMID:34302655

Abstract

Human iPSC-derived cardiomyocytes (hiPSC-CMs) are expected to be used in regenerative therapies and drug discovery for heart failure. hiPSC-CMs are a mixture of mainly ventricular CMs (VCMs) and also of atrial CMs (ACMs) and pacemaker cells. Here we describe a method to enrich VCM and ACM differentiation and to characterize these subtypes by gene expression analysis using qRT-PCR and by electrophysiological properties using the patch-clamp method. The differentiated VCMs and ACMs highly express VCM and ACM marker genes, respectively. Furthermore, both subtypes show specific properties of action potentials.

摘要

人诱导多能干细胞衍生的心肌细胞（hiPSC-CMs）有望用于心力衰竭的再生疗法和药物发现。hiPSC-CMs 主要是由心室肌细胞（VCMs）组成，也有部分心房肌细胞（ACMs）和起搏细胞。本文描述了一种通过 qRT-PCR 基因表达分析和膜片钳方法的电生理特性来富集 VCM 和 ACM 分化，并对这些亚型进行特征分析的方法。分化的 VCM 和 ACM 分别高度表达 VCM 和 ACM 标记基因。此外，这两种亚型都表现出动作电位的特定特性。