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人诱导多能干细胞（iPSC-CMs）来源的心肌细胞在聚己内酯和聚氨酯纳米纤维垫上的成熟。

Maturation of human cardiomyocytes derived from induced pluripotent stem cells (iPSC-CMs) on polycaprolactone and polyurethane nanofibrous mats.

机构信息

Chair of Medical Biotechnology, Faculty of Chemistry, Warsaw University of Technology, Noakowskiego 3, 00-664, Warsaw, Poland.

Laboratory of Cell Signaling and Metabolic Disorders, Nencki Institute of Experimental Biology PAS, Warsaw, Poland.

出版信息

Sci Rep. 2024 Jun 5;14(1):12975. doi: 10.1038/s41598-024-63905-z.

DOI:10.1038/s41598-024-63905-z
PMID:38839879
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11153585/
Abstract

Investigating the potential of human cardiomyocytes derived from induced pluripotent stem cells (iPSC-CMs) in in vitro heart models is essential to develop cardiac regenerative medicine. iPSC-CMs are immature with a fetal-like phenotype relative to cardiomyocytes in vivo. Literature indicates methods for enhancing the structural maturity of iPSC-CMs. Among these strategies, nanofibrous scaffolds offer more accurate mimicry of the functioning of cardiac tissue structures in the human body. However, further research is needed on the use of nanofibrous mats to understand their effects on iPSC-CMs. Our research aimed to evaluate the suitability of poly(ε-caprolactone) (PCL) and polyurethane (PU) nanofibrous mats with different elasticities as materials for the maturation of iPSC-CMs. Analysis of cell morphology and orientation and the expression levels of selected genes and proteins were performed to determine the effect of the type of nanofibrous mats on the maturation of iPSC-CMs after long-term (10-day) culture. Understanding the impact of 3D structural properties in in vitro cardiac models on induced pluripotent stem cell-derived cardiomyocyte maturation is crucial for advancing cardiac tissue engineering and regenerative medicine because it can help optimize conditions for obtaining more mature and functional human cardiomyocytes.

摘要

研究诱导多能干细胞（iPSC-CMs）衍生的人心肌细胞在体外心脏模型中的潜力对于开发心脏再生医学至关重要。与体内的心肌细胞相比，iPSC-CMs 不成熟，具有胎儿样表型。文献表明了增强 iPSC-CMs 结构成熟的方法。在这些策略中，纳米纤维支架更能准确模拟人体心脏组织结构的功能。然而，需要进一步研究纳米纤维垫的用途，以了解它们对 iPSC-CMs 的影响。我们的研究旨在评估具有不同弹性的聚（ε-己内酯）（PCL）和聚氨酯（PU）纳米纤维垫作为 iPSC-CMs 成熟材料的适用性。通过分析细胞形态和取向以及选定基因和蛋白质的表达水平，确定纳米纤维垫的类型对长期（10 天）培养后 iPSC-CMs 成熟的影响。了解 3D 结构特性对体外心脏模型中诱导多能干细胞衍生的心肌细胞成熟的影响对于推进心脏组织工程和再生医学至关重要，因为它可以帮助优化获得更成熟和功能更强大的人类心肌细胞的条件。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/16c7/11153585/8e547444f73e/41598_2024_63905_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/16c7/11153585/ec91f5e5808f/41598_2024_63905_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/16c7/11153585/754fb8a26ad1/41598_2024_63905_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/16c7/11153585/ea87d3717b87/41598_2024_63905_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/16c7/11153585/c777ab94a8d1/41598_2024_63905_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/16c7/11153585/a12363e2fc89/41598_2024_63905_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/16c7/11153585/8e547444f73e/41598_2024_63905_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/16c7/11153585/ec91f5e5808f/41598_2024_63905_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/16c7/11153585/754fb8a26ad1/41598_2024_63905_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/16c7/11153585/ea87d3717b87/41598_2024_63905_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/16c7/11153585/c777ab94a8d1/41598_2024_63905_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/16c7/11153585/a12363e2fc89/41598_2024_63905_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/16c7/11153585/8e547444f73e/41598_2024_63905_Fig6_HTML.jpg

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