Pandey Utsav, Szpara Moriah L
Department of Biochemistry and Molecular Biology, Center for Infectious Disease Dynamics, and The Huck Institutes of the Life Sciences, Pennsylvania State University, University Park, Pennsylvania.
Department of Pathology and Laboratory Medicine, Children's Hospital Los Angeles, Los Angeles, California.
Clin Microbiol Newsl. 2019 Mar 1;41(5):41-48. doi: 10.1016/j.clinmicnews.2019.02.003. Epub 2019 Feb 19.
Herpes simplex virus (HSV) serotypes 1 and 2 are among the most widespread human viruses. HSV disease has a complex phenotype, with symptoms that can range from mild lesions to encephalitis. In the clinical setting, this diversity of outcomes poses a major challenge, making timely disease diagnosis and treatment challenging. High-throughput sequencing (HTS) has been one of the breakthrough technologies in the modern era of molecular biology, and it is revolutionizing the study of pathogen biology and clinical diagnostics. Here, we review recent studies that have used HTS to answer questions related to the evolution of drug resistance, transmission and spread, virulence marker identification, and the design of better antiviral therapeutics for HSV. We also highlight practical considerations for handling computational analysis of HSV genomes and adoption of HTS as a routine diagnostic procedure in the clinical laboratories.
单纯疱疹病毒1型和2型是传播最为广泛的人类病毒之一。单纯疱疹病毒疾病具有复杂的表型，症状从轻微损伤到脑炎不等。在临床环境中，这种多样的结果构成了重大挑战，使得疾病的及时诊断和治疗颇具难度。高通量测序（HTS）是现代分子生物学时代的突破性技术之一，它正在彻底改变病原体生物学研究和临床诊断。在此，我们综述了近期利用高通量测序来回答与耐药性演变、传播与扩散、毒力标志物鉴定以及设计更好的抗单纯疱疹病毒抗病毒疗法相关问题的研究。我们还强调了处理单纯疱疹病毒基因组计算分析以及在临床实验室采用高通量测序作为常规诊断程序的实际注意事项。
