全球范围内 HSV-2×HSV-1 重组株的传播。
Worldwide circulation of HSV-2 × HSV-1 recombinant strains.
机构信息
Department of Medicine, University of Washington, Seattle, WA 98195, USA.
Department of Laboratory Medicine, University of Washington, Seattle, WA 98195, USA.
出版信息
Sci Rep. 2017 Mar 13;7:44084. doi: 10.1038/srep44084.
Homo sapiens harbor two distinct, medically significant species of simplexviruses, herpes simplex virus (HSV)-1 and HSV-2, with estimated divergence 6-8 million years ago (MYA). Unexpectedly, we found that circulating HSV-2 strains can contain HSV-1 DNA segments in three distinct genes. Using over 150 genital swabs from North and South America and Africa, we detected recombinants worldwide. Common, widely distributed gene UL39 genotypes are parsimoniously explained by an initial >457 basepair (bp) HSV-1 × HSV-2 crossover followed by back-recombination to HSV-2. Blocks of >244 and >539 bp of HSV-1 DNA within genes UL29 and UL30, respectively, have reached near fixation, with a minority of strains retaining sequences we posit as ancestral HSV-2. Our data add to previous in vitro and animal work, implying that in vivo cellular co-infection with HSV-1 and HSV-2 yields viable interspecies recombinants in the natural human host.
智人携带两种不同的、具有重要医学意义的单纯疱疹病毒，即单纯疱疹病毒 1 型（HSV-1）和单纯疱疹病毒 2 型（HSV-2），估计它们在 600 万至 800 万年前就已经分化。出人意料的是，我们发现循环中的 HSV-2 株可以在三个不同的基因中包含 HSV-1 DNA 片段。通过对来自北美、南美和非洲的 150 多个生殖器拭子进行检测，我们在全球范围内发现了重组病毒。常见的、广泛分布的基因 UL39 基因型可以通过最初的 HSV-1×HSV-2 交叉，跨越超过 457 个碱基对（bp），然后再重组回 HSV-2 来得到合理的解释。分别位于基因 UL29 和 UL30 中的 HSV-1 DNA 片段超过 244 和 539bp 已经接近固定，少数菌株保留了我们认为是原始 HSV-2 的序列。我们的数据增加了之前的体外和动物研究，表明在自然人类宿主中，HSV-1 和 HSV-2 的细胞共同感染会产生具有生存能力的种间重组病毒。
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