Liu Yiping, Wang Jia, Zhai Peisong, Ren Sicong, Wang Zhanqi, Peng Peixuan, Du Liuyi, Li Lisha, Zhang Yidi, Zhou Yanmin
Department of Oral Implantology, School and Hospital of Stomatology, Jilin University, Changchun 130021, China.
The Key Laboratory of Pathobiology, Ministry of Education, Norman Bethune Medical College, Jilin University, Changchun 130021, China.
Stem Cells Int. 2021 Jul 8;2021:8868004. doi: 10.1155/2021/8868004. eCollection 2021.
Recent studies, which aim to optimize maxillary sinus augmentation, have paid significant attention exploring osteogenic potential of maxillary Schneiderian sinus membrane-derived cells (MSSM-derived cells). However, it remains unclear that how MSSM-derived cells could respond to niche's biomechanical properties. Herein, this study investigated the possible effects of substrate stiffness on rMSSM-derived stem cell fate. Initially, rMSSM-derived stem cells with multiple differentiation potential were successfully obtained. We then fabricated polyacrylamide substrates with varied stiffness ranging from 13 to 68 kPa to modulate the mechanical environment of rMSSM-derived stem cells. A larger cell spreading area and increased proliferation of rMSSM-derived stem cells were found on the stiffer substrates. Similarly, cells became more adhesive as their stiffness increased. Furthermore, the higher stiffness facilitated osteogenic differentiation of rMSSM-derived stem cells. Overall, our results indicated that increase in stiffness could mediate behaviors of rMSSM-derived stem cells, which may serve as a guide in future research to design novel biomaterials for maxillary sinus augmentation.
近期旨在优化上颌窦提升的研究，已高度关注探索上颌窦施奈德膜来源细胞（MSSM来源细胞）的成骨潜能。然而，MSSM来源细胞如何响应微环境的生物力学特性仍不清楚。在此，本研究调查了底物硬度对rMSSM来源干细胞命运的可能影响。首先，成功获得了具有多种分化潜能的rMSSM来源干细胞。然后，我们制备了刚度从13至68kPa不等的聚丙烯酰胺底物，以调节rMSSM来源干细胞的力学环境。在较硬的底物上发现rMSSM来源干细胞具有更大的细胞铺展面积和更高的增殖率。同样，随着底物刚度增加，细胞的黏附性增强。此外，更高的刚度促进了rMSSM来源干细胞的成骨分化。总体而言，我们的结果表明，刚度增加可介导rMSSM来源干细胞的行为，这可为未来设计用于上颌窦提升的新型生物材料的研究提供指导。
