School of Life Sciences and Biopharmaceutics, Guangdong Pharmaceutical University, Guangzhou, China.
Guangdong Province Key Laboratory of Pharmaceutical Bioactive Substances, Guangdong Pharmaceutical University, Guangzhou, China.
J Healthc Eng. 2021 Jul 2;2021:6402206. doi: 10.1155/2021/6402206. eCollection 2021.
Atherosclerosis (AS) is a common chronic vascular inflammatory disease and one of the main causes of cardiovascular/cerebrovascular diseases (CVDs). Autophagy-related genes (ARGs) play a crucial part in pathophysiological processes of AS. However, the expression profile of ARGs has rarely been adopted to explore the relationship between autophagy and AS. Therefore, using the expression profile of ARGs to explore the relationship between autophagy and AS may provide new insights for the treatment of CVDs.
The differentially expressed ARGs of the GSE57691 dataset were obtained from the Human Autophagy Database (HADb) and the Gene Expression Omnibus (GEO) database, and the GSE57691 dataset contains 9 aortic atheroma tissues and 10 normal aortic tissues. The differentially expressed ARGs of the GSE57691 dataset were analyzed by protein-protein interaction (PPI), gene ontology analysis (GO), and Kyoto Encyclopedia of Genes and Genomes analysis (KEGG) and were chosen to explore related miRNAs/transcriptional factors.
The GSE57691 dataset had a total of 41 differentially expressed ARGs. The GO analysis results revealed that ARGs were mainly enriched in autophagy, autophagosome, and protein serine/threonine kinase activity. KEGG analysis results showed that ARGs were mainly enriched in autophagy-animal and longevity regulating signaling pathways. Expressions of ATG5, MAP1LC3B, MAPK3, MAPK8, and RB1CC1 were regarded as focus in the PPI regulatory networks. Furthermore, 11 related miRNAs and 6 related transcription factors were obtained by miRNAs/transcription factor target network analysis.
Autophagy and ARGs may play a vital role in regulating the pathophysiology of AS.
动脉粥样硬化（AS）是一种常见的慢性血管炎症性疾病，也是心血管/脑血管疾病（CVDs）的主要原因之一。自噬相关基因（ARGs）在 AS 的病理生理过程中起着至关重要的作用。然而，ARGs 的表达谱很少被用于探索自噬与 AS 之间的关系。因此，利用 ARGs 的表达谱来探索自噬与 AS 之间的关系，可能为 CVDs 的治疗提供新的思路。
从人类自噬数据库（HADb）和基因表达综合数据库（GEO）数据库中获得 GSE57691 数据集的差异表达 ARGs，该数据集包含 9 个主动脉粥样硬化组织和 10 个正常主动脉组织。通过蛋白质-蛋白质相互作用（PPI）、基因本体论分析（GO）和京都基因与基因组百科全书分析（KEGG）对 GSE57691 数据集的差异表达 ARGs 进行分析，并选择其来探索相关的 miRNA/转录因子。
GSE57691 数据集共有 41 个差异表达的 ARGs。GO 分析结果表明，ARGs 主要富集在自噬、自噬体和蛋白丝氨酸/苏氨酸激酶活性。KEGG 分析结果表明，ARGs 主要富集在自噬-动物和长寿调节信号通路。PPI 调控网络中，ATG5、MAP1LC3B、MAPK3、MAPK8 和 RB1CC1 的表达被视为重点。此外，通过 miRNA/转录因子靶标网络分析获得了 11 个相关 miRNA 和 6 个相关转录因子。
自噬和 ARGs 可能在调节 AS 的病理生理学方面发挥着重要作用。
