Micheni Lisa Nkatha, Kassaza Kennedy, Kinyi Hellen, Ntulume Ibrahim, Bazira Joel
Department of Microbiology, Mbarara University of Science and Technology, P.O. Box 1410 Mbarara, Uganda.
Department of Microbiology and Immunology, Kampala International University Western Campus, P.O. Box 71, Bushenyi, Uganda.
Tuberc Res Treat. 2021 Jun 25;2021:5588339. doi: 10.1155/2021/5588339. eCollection 2021.
Uganda is among the 22 countries in the world with a high burden of tuberculosis. The southwestern region of the country has consistently registered a high TB/HIV incidence rate. This study is aimed at characterizing the complex (MTBC) genotypic diversity in southwestern Uganda. A total of 283 sputum samples from patients with pulmonary tuberculosis were genotyped using specific single nucleotide polymorphism markers for lineages 3 and 4. Most of the patients were males with a mean age of 34. The lineage 4 Ugandan family was found to be the most dominant strains accounting for 59.7% of all cases followed by lineage 3 at 15.2%. The lineage 4 non-Ugandan family accounted for 14.5% of all cases while 4.2% showed amplification for both lineage 4 and lineage 3. Eighteen samples (6.4%) of the strains remained unclassified since they could not be matched to any lineage based on the genotyping technique used. This study demonstrates that a wide diversity of strains is causing pulmonary tuberculosis in this region with those belonging to the lineage 4 Ugandan family being more predominant. However, to confirm this, further studies using more discriminative genotyping methods are necessary.
乌干达是世界上22个结核病负担沉重的国家之一。该国西南部地区的结核病/艾滋病毒发病率一直很高。本研究旨在描述乌干达西南部结核分枝杆菌复合群（MTBC）的基因型多样性。使用针对3型和4型谱系的特定单核苷酸多态性标记，对283份肺结核患者的痰标本进行基因分型。大多数患者为男性，平均年龄34岁。发现4型乌干达家族是最主要的菌株，占所有病例的59.7%，其次是3型，占15.2%。4型非乌干达家族占所有病例的14.5%，而4.2%的病例显示4型和3型均有扩增。18份样本（6.4%）的菌株仍无法分类，因为根据所使用的基因分型技术，它们无法与任何谱系匹配。本研究表明，该地区导致肺结核的菌株种类繁多，其中4型乌干达家族的菌株更为常见。然而，要证实这一点，有必要使用更具鉴别力的基因分型方法进行进一步研究。
