Jia Yan, Cao Jinshan, Wei Zhanyong
Laboratory of Veterinary Pharmacology, College of Veterinary Medicine, Inner Mongolia Agricultural University, No. 306, Zhaowuda Road, Saihan District, 010018 Hohhot, China.
Key Laboratory of Animal-Derived Food Safety of Henan Province, Zhengzhou 450002, China.
Biomed Res Int. 2021 Jul 1;2021:6689471. doi: 10.1155/2021/6689471. eCollection 2021.
This article is aimed at analyzing the structure and function of the spike (S) proteins of porcine enteric coronaviruses, including transmissible gastroenteritis virus (TGEV), porcine epidemic diarrhea virus (PEDV), porcine deltacoronavirus (PDCoV), and swine acute diarrhea syndrome coronavirus (SADS-CoV) by applying bioinformatics methods. The physical and chemical properties, hydrophilicity and hydrophobicity, transmembrane region, signal peptide, phosphorylation and glycosylation sites, epitope, functional domains, and motifs of S proteins of porcine enteric coronaviruses were predicted and analyzed through online software. The results showed that S proteins of TGEV, PEDV, SADS-CoV, and PDCoV all contained transmembrane regions and signal peptide. TGEV S protein contained 139 phosphorylation sites, 24 glycosylation sites, and 53 epitopes. PEDV S protein had 143 phosphorylation sites, 22 glycosylation sites, and 51 epitopes. SADS-CoV S protein had 109 phosphorylation sites, 20 glycosylation sites, and 43 epitopes. PDCoV S protein had 124 phosphorylation sites, 18 glycosylation sites, and 52 epitopes. Moreover, TGEV, PEDV, and PDCoV S proteins all contained two functional domains and two motifs, spike_rec_binding and corona_S2. The corona_S2 consisted of S2 subunit heptad repeat 1 (HR1) and S2 subunit heptad repeat 2 (HR2) region profiles. Additionally, SADS-CoV S protein was predicted to contain only one functional domain, the corona_S2. This analysis of the biological functions of porcine enteric coronavirus spike proteins can provide a theoretical basis for the design of antiviral drugs.
本文旨在通过应用生物信息学方法，分析猪肠道冠状病毒的刺突（S）蛋白的结构和功能，这些病毒包括传染性胃肠炎病毒（TGEV）、猪流行性腹泻病毒（PEDV）、猪三角洲冠状病毒（PDCoV）和猪急性腹泻综合征冠状病毒（SADS-CoV）。通过在线软件对猪肠道冠状病毒S蛋白的理化性质、亲水性和疏水性、跨膜区域、信号肽、磷酸化和糖基化位点、表位、功能域和基序进行了预测和分析。结果表明，TGEV、PEDV、SADS-CoV和PDCoV的S蛋白均含有跨膜区域和信号肽。TGEV S蛋白含有139个磷酸化位点、24个糖基化位点和53个表位。PEDV S蛋白有143个磷酸化位点、22个糖基化位点和51个表位。SADS-CoV S蛋白有109个磷酸化位点、20个糖基化位点和43个表位。PDCoV S蛋白有124个磷酸化位点、18个糖基化位点和52个表位。此外，TGEV、PEDV和PDCoV的S蛋白均含有两个功能域和两个基序，即spike_rec_binding和corona_S2。corona_S2由S2亚基七肽重复序列1（HR1）和S2亚基七肽重复序列2（HR2）区域图谱组成。此外，预测SADS-CoV S蛋白仅含有一个功能域，即corona_S2。对猪肠道冠状病毒刺突蛋白生物学功能的这一分析可为抗病毒药物的设计提供理论依据。
