Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, 59 Mucai Street, Xiangfang District, Harbin 150030, China.
Animal Parasitic Diseases Laboratory, Agricultural Research Service, Beltsville, Maryland, USA.
Virology. 2014 May;456-457:20-7. doi: 10.1016/j.virol.2014.01.010. Epub 2014 Mar 25.
Three phage-displayed peptides designated H, S and F that recognize porcine aminopeptidase N (pAPN), the cellular receptor of porcine transmissible gastroenteritis virus (TGEV) were able to inhibit cell infection by TGEV. These same peptides had no inhibitory effects on infection of Vero cells by porcine epidemic diarrhea virus (PEDV). However, when PEDV, TGEV and porcine pseudorabies virus were incubated with peptide H (HVTTTFAPPPPR), only infection of Vero cells by PEDV was inhibited. Immunofluoresence assays indicated that inhibition of PEDV infection by peptide H was independent of pAPN. Western blots demonstrated that peptide H interacted with PEDV spike protein and that pre-treatment of PEDV with peptide H led to a higher inhibition than synchronous incubation with cells. These results indicate direct interaction with the virus is necessary to inhibit infectivity. Temperature shift assays demonstrated that peptide H inhibited pre-attachment of the virus to the cells.
三种噬菌体展示肽 H、S 和 F 可识别猪氨基肽酶 N（pAPN），即猪传染性胃肠炎病毒（TGEV）的细胞受体，能抑制 TGEV 对细胞的感染。这些相同的肽对猪流行性腹泻病毒（PEDV）感染 Vero 细胞没有抑制作用。然而，当 PEDV、TGEV 和猪伪狂犬病病毒与肽 H（HVTTTFAPPPPR）孵育时，只有 PEDV 感染 Vero 细胞被抑制。免疫荧光分析表明，肽 H 抑制 PEDV 感染不依赖于 pAPN。Western blot 表明肽 H 与 PEDV 刺突蛋白相互作用，并且肽 H 预处理 PEDV 比与细胞同步孵育导致更高的抑制率。这些结果表明与病毒的直接相互作用是抑制感染性所必需的。温度转移试验表明肽 H 抑制了病毒与细胞的预附着。
