miR-101-3p 作为肾细胞癌的肿瘤抑制因子，通过靶向 EZH2 抑制其侵袭和转移。

miR-101-3p Serves as a Tumor Suppressor for Renal Cell Carcinoma and Inhibits Its Invasion and Metastasis by Targeting EZH2.

机构信息

Department of Urology, Shanghai Tenth People's Hospital, School of Medicine in Tongji University, 301 Yanchang Road, Jing'an District, Shanghai 200072, China.

出版信息

Biomed Res Int. 2021 Jul 7;2021:9950749. doi: 10.1155/2021/9950749. eCollection 2021.

BACKGROUND

The role of miRNAs in renal cell carcinoma (RCC) is not certain. We wanted to study the biological functions and potential mechanisms of miR-101-3p in RCC.

METHODS

miR-101-3p was inhibited in A498 and OSRC-2 (two RCC cell lines). We studied its effect on cell invasion and proliferation. Target EZH2 of miR-101-3p was designated by different methods, including luciferase functional analysis and Western blotting. The expression level of the target gene in treated cells was quantitatively analyzed by quantitative real-time polymerase chain reaction. In addition, induction of miR-101-3p to prevent tumor formation of A498 cells in mice was further studied.

RESULTS

The overexpression of miR-101-3p significantly inhibited the proliferation, migration, and invasion in two RCC cells. Western blotting and luciferase functional analysis indicated that miR-101-3p regulated the expression of EZH2 in two cell lines. Mice inoculated with A498 and OSRC-2 cells transfected with miR-101-3p mimics showed significantly smaller xenografts and weaker EZH2 expression levels than the control group.

CONCLUSIONS

miR-101-3p inhibited RCC cell proliferation, migration, and invasion by targeting EZH2.

背景

miRNAs 在肾细胞癌（RCC）中的作用尚不确定。我们希望研究 miR-101-3p 在 RCC 中的生物学功能和潜在机制。

方法

在 A498 和 OSRC-2（两种 RCC 细胞系）中抑制 miR-101-3p。我们研究了它对细胞侵袭和增殖的影响。通过不同的方法确定 miR-101-3p 的靶基因 EZH2，包括荧光素酶功能分析和 Western blot。通过定量实时聚合酶链反应定量分析处理细胞中靶基因的表达水平。此外，进一步研究了 miR-101-3p 诱导 A498 细胞肿瘤形成的能力。

结果

miR-101-3p 的过表达显著抑制了两种 RCC 细胞的增殖、迁移和侵袭。Western blot 和荧光素酶功能分析表明，miR-101-3p 调节两种细胞系中 EZH2 的表达。与对照组相比，接种 miR-101-3p 模拟物转染的 A498 和 OSRC-2 细胞的小鼠异种移植物明显较小，EZH2 表达水平较弱。