Stomatology Hospital, School of Stomatology, Zhejiang University School of Medicine, Clinical Research Center for Oral Diseases of Zhejiang Province, Key Laboratory of Oral Biomedical Research of Zhejiang Province, Cancer Center of Zhejiang University, Hangzhou 310006, China.
Biomed Res Int. 2021 Jul 9;2021:9978651. doi: 10.1155/2021/9978651. eCollection 2021.
Temporomandibular joint osteoarthritis (TMJOA) is characterized by chronic inflammatory degradation of mandibular condylar cartilage (MCC). Studies have found a positive correlation between inflammation and cyclooxygenase- (COX-) 2 in OA pathology. NF-B is a crucial transcription factor of inflammatory and immune responses in the cause of TMJOA pathology. Resveratrol (RES) plays a critical role in antioxidation and anti-inflammation. But, studies on the effects of RES on TMJOA are very limited. So, the purpose of this study is to investigate the antioxidant and protective effects of RES against MCC degradation through downregulating COX-2/NF-B expression. In vitro studies, the MCC cells were divided into three groups: the NC group, OA group, and RES group. The optimum dose of RES (10 M) was determined. The TMJOA model of mice was created by injection of collagenase. And mice were injected with RES (100 g/10 l) 3 times one week for 4 weeks in the RES group. The expressions of COX-2, P65, MMP1, MMP13, COL2, and ACAN were measured by RT-PCR. Morphological changes of MCC were studied with HE staining. The results showed that inflammation could induce MCC degradation in vitro and vivo, while RES could reverse the degradation. Meanwhile, RES could downregulate COX-2/NF-B/MMP expression and increase cartilage markers in vitro and vivo studies. The results indicated that RES treatment had antioxidant effects against chondrocyte apoptosis by downregulating the COX-2/NF-B pathway in created TMJOA.
颞下颌关节骨关节炎（TMJOA）的特征是慢性炎症性下颌髁突软骨（MCC）降解。研究发现炎症与 OA 病理学中的环氧化酶-（COX-）2 之间存在正相关。NF-B 是 TMJOA 病理学中炎症和免疫反应的关键转录因子。白藜芦醇（RES）在抗氧化和抗炎中起着至关重要的作用。但是，关于 RES 对 TMJOA 影响的研究非常有限。因此，本研究旨在通过下调 COX-2/NF-B 表达来研究 RES 对 MCC 降解的抗氧化和保护作用。体外研究中，将 MCC 细胞分为三组：NC 组、OA 组和 RES 组。确定了 RES（10μM）的最佳剂量。通过注射胶原酶建立 TMJOA 小鼠模型。RES 组每周注射 RES（100μg/10l）3 次，共 4 周。通过 RT-PCR 测量 COX-2、P65、MMP1、MMP13、COL2 和 ACAN 的表达。用 HE 染色研究 MCC 的形态变化。结果表明，炎症可在体外和体内诱导 MCC 降解，而 RES 可逆转降解。同时，RES 可下调 COX-2/NF-B/MMP 表达，并增加体外和体内研究中的软骨标志物。结果表明，RES 治疗通过下调 COX-2/NF-B 通路对 TMJOA 中软骨细胞凋亡具有抗氧化作用。
