通过血清分析的肽微阵列对 SARS-CoV-2 刺突线性表位进行扫描。
SARS-CoV-2 spike linear epitope scanning via a peptide microarray through sera profiling.
机构信息
Shanghai Center for Systems Biomedicine, Key Laboratory of Systems Biomedicine (Ministry of Education), Shanghai Jiao Tong University, Shanghai, China.
College of Life Science, Nankai University, Tianjin 300071, China.
出版信息
STAR Protoc. 2021 Sep 17;2(3):100707. doi: 10.1016/j.xpro.2021.100707. Epub 2021 Jul 10.
Host humoral immunological response plays an essential role in protection against pathogens. Identification of B-cell epitopes on antigens is required for accurate diagnosis and vaccine development. To map SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2) spike linear epitopes, we developed a protocol of profiling sera from patients with COVID-19 (coronavirus disease 2019) via a peptide microarray designed according to spike protein. The protocol is also applicable for other antigens or sample types. This protocol is rapid, high throughput, and the cost is acceptable while it needs specialized microarray facilities. For complete details on the use and execution of this protocol, please refer to Li et al. (2020, 2021a, 2021b).
宿主体液免疫应答在抵御病原体方面起着至关重要的作用。鉴定抗原上的 B 细胞表位是准确诊断和疫苗开发的需要。为了绘制 SARS-CoV-2（严重急性呼吸综合征冠状病毒 2）刺突线性表位图谱，我们根据刺突蛋白设计了一种肽微阵列，开发了一种用于分析 COVID-19（2019 年冠状病毒病）患者血清的方案。该方案也适用于其他抗原或样本类型。该方案快速、高通量，成本可以接受，同时需要专门的微阵列设备。有关该方案的使用和执行的完整详细信息，请参阅 Li 等人（2020 年，2021a 年，2021b 年）。
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