Miki K, Itoh T, Nose H, Tanaka Y, Morimoto T
Department of Physiology, Kyoto Prefectural University of Medicine, Japan.
Jpn J Physiol. 1987;37(4):687-98. doi: 10.2170/jjphysiol.37.687.
Changes in hematocrit and plasma oncotic pressure were measured continuously during and after isotonic volume expansion in splenectomized dogs in order to test the potential of these types of measurements to predict changes in plasma volume. A volume of Ringer's solution amounting to 15% of the initial blood volume was infused over 10 min. At the end of the infusion, 54% of infused fluid remained within the intravascular space and 9% of the infused volume was retained within the intravascular space at 50 min after the end of the infusion. Hematocrit and plasma oncotic pressure decreased by 2.4% and 2.4 mmHg, respectively, at the end of infusion and then variables returned to their control levels gradually. Changes in plasma volume were estimated indirectly from hematocrit and plasma oncotic pressure based on the dilution of the erythrocytes and the protein. Highly significant correlations were observed between the measured plasma volume (Y) and the plasma volume (X) calculated from hematocrit (Y = 0.95X + 0.22, r = 0.96) and the plasma oncotic pressure (Y = 0.86X + 0.46, r = 0.91). We therefore conclude that either hematocrit and plasma oncotic pressure measured continuously are reliable parameters for predicting the time course of the plasma volume change during an isotonic volume expansion of up to 15% of the initial blood volume.
在脾切除的狗进行等渗容量扩张期间及之后，连续测量血细胞比容和血浆胶体渗透压，以测试这些类型的测量对于预测血浆容量变化的潜力。在10分钟内输注相当于初始血容量15%的林格氏溶液。输注结束时，54%的输注液保留在血管内空间，输注结束后50分钟时，9%的输注量保留在血管内空间。输注结束时，血细胞比容和血浆胶体渗透压分别下降2.4%和2.4 mmHg，然后这些变量逐渐恢复到对照水平。基于红细胞和蛋白质的稀释，根据血细胞比容和血浆胶体渗透压间接估计血浆容量变化。在测量的血浆容量（Y）与根据血细胞比容计算的血浆容量（X）（Y = 0.95X + 0.22，r = 0.96）以及血浆胶体渗透压（Y = 0.86X + 0.46，r = 0.91）之间观察到高度显著的相关性。因此，我们得出结论，连续测量的血细胞比容和血浆胶体渗透压是预测初始血容量高达15%的等渗容量扩张期间血浆容量变化时间进程的可靠参数。
