Chen Zhao-Jin, Lin Li-An, Li Ying-Jun, Chen Yan, Zhang Hao, Han Hui, Wu Nai-Cheng, Nicola Fohrer, Li Yu-Ying, Ren Xue-Min
International Joint Laboratory of Watershed Ecological Security and Collaborative Innovation Center of Water Security for Water Source Region of Middle Route Project of South-North Water Diversion in Henan Province, School of Water Resources and Environmental Engineering, Nanyang Normal University, Nanyang 473061, China.
School of Life Science and Agricultural Engineering, Nanyang Normal University, Nanyang 473061, China.
Huan Jing Ke Xue. 2021 Aug 8;42(8):3997-4004. doi: 10.13227/j.hjkx.202011198.
As a representative of second-generation bioenergy plants, has received increasing attention in the studies of heavy metal (HM)-contaminated soil remediation. Currently, few studies have examined the effects of using to remediate HM-contaminated soils on the composition and function of microbial communities. In this study, the cultivar was examined for its tolerance and enrichment abilities when grown in soils containing 100 mg ·kg of cadmium (Cd). The structure, function, and co-occurrence network of their rhizosphere bacterial communities were analyzed during the remediation process. MiSeq sequencing showed that the rhizosphere bacterial community comprised 32 phyla and 425 genera, including plant growth-promoting rhizobacteria (PGPR), such as , , , and . The addition of Cd affected the rhizosphere bacterial community and reduced community diversity. Phylogenetic molecular ecological networks indicated that Cd addition reduced the interactions between rhizosphere bacteria to generate a simpler network structure, increased the number of negative-correlation links, enhanced the competition between rhizosphere bacterial species, and changed the composition of key bacteria. PICRUSt functional predictive analysis indicated that Cd stress reduced soil bacterial functions in the rhizosphere. The results of this study provide a reference for the subsequent regulation of efficient remediation by PGPRs or key bacteria.
作为第二代生物能源植物的代表，在重金属（HM）污染土壤修复研究中受到越来越多的关注。目前，很少有研究考察利用其修复HM污染土壤对微生物群落组成和功能的影响。在本研究中，检测了该品种在含100 mg·kg镉（Cd）的土壤中生长时的耐受性和富集能力。在修复过程中分析了其根际细菌群落的结构、功能和共生网络。MiSeq测序表明，该植物根际细菌群落包含32个门和425个属，包括促进植物生长的根际细菌（PGPR），如、、和。添加Cd影响了该植物根际细菌群落并降低了群落多样性。系统发育分子生态网络表明，添加Cd减少了该植物根际细菌之间的相互作用，产生了更简单的网络结构，增加了负相关连接的数量，增强了根际细菌物种之间的竞争，并改变了关键细菌的组成。PICRUSt功能预测分析表明，Cd胁迫降低了该植物根际土壤细菌的功能。本研究结果为后续通过PGPR或关键细菌调控高效修复提供了参考。
