Innovation Center of Water Security for Water Source Region of Mid-route Project of South-North Water Diversion of Henan Province, Henan Engineering Technology Research Center for Mushroom-based Foods, School of Agricultural Engineering, Nanyang Normal University, Nanyang, 473061, China.
Nanjing Institute of Environmental Sciences, Ministry of Ecology and Environment, Nanjing, 210042, China.
Arch Microbiol. 2020 Jul;202(5):1157-1171. doi: 10.1007/s00203-020-01830-1. Epub 2020 Feb 17.
Soil microbes play important roles in plant growth and in the biogeochemical cycling of earth's elements. However, the structure and functions of the microbial community associated with the growth of second-generation energy crops, such as Miscanthus, remain unclear. Thus, in this study, the composition and function of the bacterial and fungal communities associated with Miscanthus cultivation were analyzed by MiSeq sequencing combined with PICRUSt and FUNGUIld analyses. The results of community composition and diversity index analyses showed that Miscanthus cultivation significantly altered the bacterial and fungal community composition and reduced bacterial and fungal diversity. In addition, Miscanthus cultivation increased the soil organic matter (SOM) and total nitrogen (TN) contents. The correlation analysis between microbial community composition and environmental factors indicated that SOM and TN were the most important factors affecting bacterial and fungal communities. Miscanthus cultivation could enrich the abundances of Pseudomonas, Rhizobium, Luteibacter, Bradyrhizobium, Phenylobacterium and other common plant-promoting bacteria, while also increasing Cladophialophora, Hymenula, Magnaporthe, Mariannaea, etc., which predicted corresponded to the saprotrophic, plant pathogenic, and pathotrophic trophic modes. The PICRUSt predictive analysis indicated that Miscanthus cultivation altered the metabolic capabilities of bacterial communities, including the metabolism of carbon, nitrogen, and phosphorus cycle. In addition, FUNGUIld analysis indicated that Miscanthus cultivation altered the fungal trophic mode. The effects of Miscanthus on the communities and function of bacteria and fungi varied among Miscanthus species. Miscanthus specie Xiangdi NO 1 had the greatest impact on soil bacterial and fungal communities, whereas Miscanthus specie Wujiemang NO 1 had the greatest impact on soil bacteria and fungi functions. The results of this study provide a reference for the composition and function of microbial communities during the growth of Miscanthus.
土壤微生物在植物生长和地球元素的生物地球化学循环中发挥着重要作用。然而，与第二代能源作物（如芒属植物）生长相关的微生物群落的结构和功能仍不清楚。因此，本研究通过 MiSeq 测序结合 PICRUSt 和 FUNGUIld 分析，分析了与芒属植物栽培相关的细菌和真菌群落的组成和功能。群落组成和多样性指数分析的结果表明，芒属植物栽培显著改变了细菌和真菌群落的组成，降低了细菌和真菌的多样性。此外，芒属植物栽培增加了土壤有机质（SOM）和总氮（TN）含量。微生物群落组成与环境因子的相关性分析表明，SOM 和 TN 是影响细菌和真菌群落的最重要因素。芒属植物栽培可以富集 Pseudomonas、Rhizobium、Luteibacter、Bradyrhizobium、Phenylobacterium 等常见的植物促生细菌，同时增加 Cladophialophora、Hymenula、Magnaporthe、Mariannaea 等，预测与腐生、植物病原和病理营养型相对应。PICRUSt 预测分析表明，芒属植物栽培改变了细菌群落的代谢能力，包括碳、氮和磷循环的代谢。此外，FUNGUIld 分析表明，芒属植物栽培改变了真菌的营养模式。芒属植物对细菌和真菌群落的影响因芒属植物的种类而异。芒属植物 Xiangdi NO 1 对土壤细菌和真菌群落的影响最大，而芒属植物 Wujiemang NO 1 对土壤细菌和真菌功能的影响最大。本研究结果为芒属植物生长过程中微生物群落的组成和功能提供了参考。
