Zhang Dongdong, Qi Dongxu, Xu Yi, Hu Chunhe, Zhang Xiao, Yang Qingjian, Shang Zikun, Zhang Guisheng
Department of Hand Surgery, The Third Hospital of Hebei Medical University, Shijiazhuang, Hebei, 050051, People's Republic of China.
Can J Physiol Pharmacol. 2021 Dec;99(12):1280-1287. doi: 10.1139/cjpp-2021-0006. Epub 2021 Jul 26.
Skin flap transfer is an important method to repair and reconstruct various tissue defects; however, avascular necrosis largely affects the success of flap transfer. The sphingosine 1-phosphate receptor 1 (S1PR1) agonist SEW2871 has been proven to ameliorate ischemic injury; however, its effect on flap survival has not been reported. In this study, an experimental skin flap model was established in rats to investigate the roles of SEW2871. The results indicated that SEW2871 greatly increased the survival of the skin flap, alleviated pathological injury, promoted the angiogenesis, and inhibited cells apoptosis in skin flap tissues. SEW2871 activated S1PR1 downstream signaling pathways, including heat shock protein 27 (HSP27), extracellular regulated protein kinases (ERK), and protein kinase B (Akt). In addition, SEW2871 promoted the expression of S1PR1. These findings may provide novel insights for skin flap transfer.
皮瓣移植是修复和重建各种组织缺损的重要方法；然而，缺血性坏死在很大程度上影响皮瓣移植的成功率。1-磷酸鞘氨醇受体1（S1PR1）激动剂SEW2871已被证明可改善缺血性损伤；然而，其对皮瓣存活的影响尚未见报道。在本研究中，建立大鼠实验性皮瓣模型以研究SEW2871的作用。结果表明，SEW2871可显著提高皮瓣存活率，减轻病理损伤，促进血管生成，并抑制皮瓣组织中的细胞凋亡。SEW2871激活了S1PR1下游信号通路，包括热休克蛋白27（HSP27）、细胞外调节蛋白激酶（ERK）和蛋白激酶B（Akt）。此外，SEW2871促进了S1PR1的表达。这些发现可能为皮瓣移植提供新的见解。
