Alvarez de Laviada T, Romero F J, Antón V, Antón V, Romá J, Viña J, Sáez G T
Department of Physiology, Faculty of Medicine, University of Valencia, Spain.
J Anal Toxicol. 1987 Nov-Dec;11(6):260-2. doi: 10.1093/jat/11.6.260.
A simple microassay for the determination of hydrazine in laboratory samples is presented. The colored product of the reaction of p-dimethylaminobenzaldehyde with hydrazine was tested at 470 nm using double-beam mode in different samples. Internal standards and data on blood serum, liver, and brain of rats treated with hydrazine or isoniazid are presented. The tissue glutathione content of these rats was determined, and the possible implication of glutathione in the brain toxicity of hydrazine is discussed.
本文介绍了一种用于测定实验室样品中肼的简单微量测定法。在不同样品中，采用双光束模式在470nm波长下检测对二甲氨基苯甲醛与肼反应生成的有色产物。给出了用肼或异烟肼处理的大鼠血清、肝脏和大脑的内标及数据。测定了这些大鼠组织中的谷胱甘肽含量，并讨论了谷胱甘肽在肼脑毒性中的可能作用。
