Romero F J, Monsalve E, Hermenegildo C, Puertas F J, Higueras V, Nies E, Segura-Aguilar J, Romá J
Department of Physiology, Universitat de València, Spain.
Neurochem Res. 1991 Feb;16(2):157-61. doi: 10.1007/BF00965704.
Nervous tissue, central and peripheral, is, as any other, subject to variations in oxygen tension, and to the attack of different xenobiotics; these situations may promote the generation of activated oxygen species of free radical character. Results are presented showing that the content of total glutathione (GSH) in brain is 10-fold that found in the sciatic nerve of the rat (2620 vs. 261 nmol/g wet weight, respectively). The existence of a relatively high superoxide dismutase activity in peripheral nervous tissue, when compared with brain or liver, in combination with the DT-diaphorase activity detected in the sciatic nerve might represent an effective defense mechanism against quinone toxicity, as is also discussed. Nervous tissue, both central and peripheral lack Se-independent GSH peroxidase activity. Finally, the activities of other glutathione-related enzymes studied in the sciatic nerve are very low, when compared with the central nervous tissue, thus suggesting a higher susceptibility of peripheral tissue to oxidative stress damage, since GSH concentration and/or any GSH-related enzymatic activities, e.g. GSH peroxidase or glutathione disulfide reductase, might become limiting.
与其他组织一样，中枢和外周神经组织也会受到氧张力变化以及不同异源生物物质的攻击；这些情况可能会促使具有自由基特征的活性氧物种的产生。研究结果表明，大鼠脑内总谷胱甘肽（GSH）的含量是坐骨神经中含量的10倍（分别为2620和261 nmol/g湿重）。与脑或肝脏相比，外周神经组织中存在相对较高的超氧化物歧化酶活性，同时在坐骨神经中检测到的DT-黄递酶活性，可能代表了一种对抗醌毒性的有效防御机制，这一点也将进行讨论。中枢和外周神经组织均缺乏不依赖硒的谷胱甘肽过氧化物酶活性。最后，与中枢神经组织相比，在坐骨神经中研究的其他与谷胱甘肽相关的酶的活性非常低，这表明外周组织对氧化应激损伤的易感性更高，因为谷胱甘肽浓度和/或任何与谷胱甘肽相关的酶活性，如谷胱甘肽过氧化物酶或谷胱甘肽二硫化物还原酶，可能会成为限制因素。
