基于 TMT 标记定量蛋白质组学的超声解冻快速冷冻小黄鱼（Larimichthys polyactis）的效果。
Effects of ultrasound on the thawing of quick-frozen small yellow croaker (Larimichthys polyactis) based on TMT-labeled quantitative proteomic.
机构信息
School of Food & Biological Engineering, Institute of Food Physical Processing, Jiangsu University, Zhenjiang 212013, China.
School of Food & Biological Engineering, Institute of Food Physical Processing, Jiangsu University, Zhenjiang 212013, China.
出版信息
Food Chem. 2022 Jan 1;366:130600. doi: 10.1016/j.foodchem.2021.130600. Epub 2021 Jul 15.
The effect of dual-frequency sequential ultrasonic thawing (DUT) on the quality of quick-frozen small yellow croaker was studied by TMT-labeled quantitative proteomic method. A total number of 75 proteins were identified as differentially abundant proteins (DAPs) in fish meat treated by DUT, while 72 DAPs were in flow water thawing (FWT). The Kyoto encyclopedia of genes and genomes (KEGG) pathway analysis indicated that the DAPs in the significant enrichment pathway of DUT group were enzymes. Compared with FWT, DUT had a significant effect on the enzyme content. The correlation analyses indicated that 40 DAPs were related with the quality traits. The 11 highly correlated DAPs are expected to be used as potential protein markers for texture profile analyses, color, thawing loss, water-holding capacity, and pH of thawed small yellow croaker quality. These results provide a further understanding of the quality stability of quick-frozen small yellow croaker treated by the DUT.
采用 TMT 标记定量蛋白质组学方法研究了双频顺序超声解冻（DUT）对速冻小黄鱼品质的影响。在 DUT 处理的鱼肉中鉴定出了 75 种差异丰度蛋白（DAP），而在流水解冻（FWT）中鉴定出了 72 种 DAP。京都基因与基因组百科全书（KEGG）途径分析表明，DUT 组显著富集途径中的 DAP 是酶。与 FWT 相比，DUT 对酶含量有显著影响。相关性分析表明，40 种 DAP 与品质特性有关。11 种高度相关的 DAP 有望用作质地分析、颜色、解冻损失、持水能力和 pH 值的潜在蛋白质标记物，以评估解冻小黄鱼的品质。这些结果为进一步了解 DUT 处理的速冻小黄鱼的品质稳定性提供了依据。
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