Zhang Xuguang, Tang Xianming, Xu Jianan, Zheng Yueping, Lin Jun, Zou Huafeng
Engineering Technology Research Center of Marine Ranching, College of Marine Ecology and Environment, Shanghai Ocean University, Shanghai, 201306, China.
Hainan Provincial Key Laboratory of Tropical Maricultural Technology, Hainan Academy of Ocean and Fisheries Sciences, Haikou, Hainan, 571126, China.
Mar Environ Res. 2024 Feb;194:106299. doi: 10.1016/j.marenvres.2023.106299. Epub 2023 Dec 14.
Noise pollution is increasingly prevalent in aquatic ecosystems, causing detrimental effects on growth and behavior of marine fishes. The physiological responses of fish to underwater noise are poorly understood. In this study, we used RNA-sequencing (RNA-seq) to study the transcriptome of the sonic muscle in small yellow croaker (Larimichthys polyactis) after exposure to a 120 dB noise for 30 min. The behavioral experiment revealed that noise exposure resulted in accelerated tail swimming behavior at the beginning of the exposure period, followed by loss of balance at the end of experiment. Transcriptomic analysis found that most highly expressed genes in the sonic muscle, including parvalbumin, slc25a4, and troponin C were related with energy metabolism and locomotor function. Further, a total of 1261 differentially expressed genes (DEGs) were identified, including 284 up-regulated and 977 down-regulated genes in the noise exposure group compared with the control group. Gene ontology (GO) analysis indicated that the most enriched categories of DEGs included protein folding and response to unfolding protein. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis found over-represented pathways including protein processing in the endoplasmic reticulum, chaperones and folding catalysts, as well as arginine and proline metabolism. Specifically, many genes related to fatty acid and collagen metabolism were up-regulated in the noise exposure group. Taken together, our results indicate that exposure to noise stressors alters the swimming behavior of croaker, inducing endoplasmic reticulum stress, disrupting lipid metabolism, and causing collagen degradation in the sonic muscle of L. polyactis.
噪声污染在水生生态系统中日益普遍，对海洋鱼类的生长和行为产生有害影响。鱼类对水下噪声的生理反应尚不清楚。在本研究中，我们使用RNA测序（RNA-seq）来研究小黄鱼（Larimichthys polyactis）在暴露于120分贝噪声30分钟后声肌的转录组。行为实验表明，噪声暴露在暴露期开始时导致尾部游泳行为加速，随后在实验结束时出现平衡丧失。转录组分析发现，声肌中大多数高表达基因，包括小清蛋白、溶质载体家族25成员4和肌钙蛋白C，都与能量代谢和运动功能有关。此外，共鉴定出1261个差异表达基因（DEG），与对照组相比，噪声暴露组中有284个上调基因和977个下调基因。基因本体（GO）分析表明，DEG最富集的类别包括蛋白质折叠和对未折叠蛋白的反应。京都基因与基因组百科全书（KEGG）通路分析发现，过表达的通路包括内质网中的蛋白质加工、伴侣蛋白和折叠催化剂，以及精氨酸和脯氨酸代谢。具体而言，噪声暴露组中许多与脂肪酸和胶原蛋白代谢相关的基因上调。综上所述，我们的结果表明，暴露于噪声应激源会改变小黄鱼的游泳行为，诱导内质网应激，扰乱脂质代谢，并导致小黄鱼声肌中的胶原蛋白降解。
