Center for Eco-Environment Restoration of Hainan Province & Key Laboratory of A&F Environmental Processes and Ecological Regulation of Hainan Province, College of Environment and Ecology, Hainan University, Renmin Ave. 58, Haikou 570228, China; State Key Laboratory of Marine Resource Utilization in South China Sea, Hainan University, Haikou 570228, China.
Center for Eco-Environment Restoration of Hainan Province & Key Laboratory of A&F Environmental Processes and Ecological Regulation of Hainan Province, College of Environment and Ecology, Hainan University, Renmin Ave. 58, Haikou 570228, China; College of Tropical Crops, Hainan University, Haikou 570228, China.
Sci Total Environ. 2021 Nov 25;797:148942. doi: 10.1016/j.scitotenv.2021.148942. Epub 2021 Jul 16.
Paclobutrazol is a triazole plant growth regulator with a wide range of applications in crop and fruit tree production. Paclobutrazol is used as a racemic mixture in agriculture. However, the effects of paclobutrazol enantiomers on soil microbial community structure and diversity are unclear. In the present study, Illumina high-throughput sequencing was used to study the enantioselective effects of two paclobutrazol enantiomers on soil microbial community. S-paclobutrazol was more persistent than R-paclobutrazol. The half-lives of the S- and R-isomers were 80 d and 50 d, respectively. No interconversion between the two isomers occurred in soils. In addition, the enantiomers had significant enantiomeric effects on soil microbial community and the paclobutrazol degradation was probably attributed to the presence of Pseudomonas and Mycobacterium. Notably, the relative abundance of Fusarium, a genus of filamentous fungi producing gibberellins, could be enantioselectively affected by the chiral enantiomers. Paclobutrazol enantiomers exhibited greater effects on the fungal community structure than bacterial community structure due to the fungicidal activity of paclobutrazol. Finally, R-paclobutrazol had a significant effect on the microbial networks. The findings of the present study suggest that the use of S-paclobutrazol may accomplish both plant growth regulation and the minimization of effects of paclobutrazol on soil microbial communities.
多效唑是一种三唑类植物生长调节剂，在作物和果树上的应用广泛。多效唑在农业中作为外消旋混合物使用。然而，多效唑对土壤微生物群落结构和多样性的影响尚不清楚。本研究采用 Illumina 高通量测序技术研究了两种多效唑对土壤微生物群落的对映体选择性影响。S-多效唑比 R-多效唑更持久。S-和 R-对映体的半衰期分别为 80 d 和 50 d，两种对映体在土壤中均未发生相互转化。此外，对映体对土壤微生物群落具有显著的对映体选择性效应，多效唑的降解可能归因于假单胞菌和分枝杆菌的存在。值得注意的是，镰刀菌的相对丰度（一种产生赤霉素的丝状真菌属）可能受到手性对映体的选择性影响。由于多效唑的杀菌活性，多效唑对真菌群落结构的影响大于细菌群落结构。最后，R-多效唑对微生物网络有显著影响。本研究结果表明，使用 S-多效唑既能实现植物生长调节，又能最大限度地减少多效唑对土壤微生物群落的影响。
