Department of Plant Protection, College of Agriculture, Northeast Agricultural University, Harbin 150030, People's Republic of China.
Phytopathology. 2022 Feb;112(2):335-344. doi: 10.1094/PHYTO-05-21-0213-R. Epub 2022 Feb 4.
does not infect nonhost maize () but infects nonhost common bean () under inoculation. Soybean seed exudates participate in mediating host resistance to . before infection. This study aims to elucidate the role of seed exudates in mediating the nonhost resistance of maize and common bean to . before infection. The behaviors of . zoospores in response to the seed exudates were determined using an assay chamber and a concave slide. The proteomes of . zoospores in response to the seed exudates were analyzed with the tandem mass tag method. The key proteins were quantitatively verified by parallel reaction monitoring. Maize seed exudates exerted a repellent effect on zoospores of . . This result explains why zoospores sense repelling signaling molecules in maize seed exudates that weaken and strongly inhibit chemotaxis signals in the phosphatidylinositol signaling pathway and arachidonic acid metabolism pathway. Common bean seed exudates did not exhibit any attraction to the zoospores because the guanine nucleotide-binding protein signaling pathway, which is responsible for transmitting chemotactic signals, had no significant change. The proteins protecting the cell membrane structure were significantly downregulated, and the early apoptosis signal glutathione was enhanced in zoospores responding to common bean seed exudates, which resulted in dissolution of the cysts. Maize and common bean seed exudates mediate part of the nonhost resistance to . via different mechanisms before infection. The immunity of maize to . is caused by the repellent effect of maize seed exudates on zoospores. Common bean seed exudates participate in mediating nonhost resistance by dissolving the cysts.
不感染非寄主玉米（），但在接种下感染非寄主普通菜豆（）。大豆种子分泌物参与调节寄主对的抗性。在感染前。本研究旨在阐明种子分泌物在调节玉米和普通菜豆对的非寄主抗性中的作用。使用培养皿和凹面载玻片测定游动孢子对种子分泌物的行为。用串联质量标签法分析游动孢子对种子分泌物的蛋白质组。通过平行反应监测定量验证关键蛋白。玉米种子分泌物对游动孢子表现出排斥作用。这一结果解释了为什么游动孢子在玉米种子分泌物中感知到削弱并强烈抑制磷脂酰肌醇信号通路和花生四烯酸代谢通路中趋化信号的排斥信号分子。普通菜豆种子分泌物对游动孢子没有任何吸引力，因为负责传递趋化信号的鸟嘌呤核苷酸结合蛋白信号通路没有明显变化。对普通菜豆种子分泌物作出反应的游动孢细胞膜结构保护蛋白显著下调，早期凋亡信号谷胱甘肽增强，导致胞囊溶解。玉米和普通菜豆种子分泌物通过不同机制在感染前介导对的部分非寄主抗性。玉米对的免疫力是由玉米种子分泌物对游动孢子的排斥作用引起的。普通菜豆种子分泌物通过溶解胞囊参与调节非寄主抗性。
