Hahamovich Evgeny, Monin Sagi, Hazan Yoav, Rosenthal Amir
Technion - Israel Institute of Technology, Haifa, Israel.
Nat Commun. 2021 Jul 26;12(1):4516. doi: 10.1038/s41467-021-24850-x.
Optical imaging is commonly performed with either a camera and wide-field illumination or with a single detector and a scanning collimated beam; unfortunately, these options do not exist at all wavelengths. Single-pixel imaging offers an alternative that can be performed with a single detector and wide-field illumination, potentially enabling imaging applications in which the detection and illumination technologies are immature. However, single-pixel imaging currently suffers from low imaging rates owing to its reliance on configurable spatial light modulators, generally limited to 22 kHz rates. We develop an approach for rapid single-pixel imaging which relies on cyclic patterns coded onto a spinning mask and demonstrate it for in vivo imaging of C. elegans worms. Spatial modulation rates of up to 2.4 MHz, imaging rates of up to 72 fps, and image-reconstruction times of down to 1.5 ms are reported, enabling real-time visualization of dynamic objects.
光学成像通常使用相机和宽场照明，或者使用单个探测器和扫描准直光束来进行；不幸的是，这些方法并非在所有波长下都可行。单像素成像提供了一种替代方案，它可以使用单个探测器和宽场照明来执行，这有可能实现检测和照明技术尚不成熟的成像应用。然而，由于依赖可配置的空间光调制器，单像素成像目前的成像速率较低，通常限制在22kHz的速率。我们开发了一种快速单像素成像方法，该方法依赖于编码在旋转掩模上的循环图案，并在秀丽隐杆线虫的体内成像中进行了演示。报告显示，空间调制速率高达2.4MHz，成像速率高达72fps，图像重建时间低至1.5ms，能够实时可视化动态物体。
