CEMBIO (Center for Metabolomics and Bioanalysis), Facultad de Farmacia, Universidad San Pablo CEU, CEU Universities, Campus Monteprincipe, Boadilla del Monte, Madrid, Spain.
Unidad Universitaria de Secuenciación Masiva y Bioinformática, Instituto de Biotecnología, Cuernavaca, Mexico.
Methods Mol Biol. 2021;2369:217-249. doi: 10.1007/978-1-0716-1681-9_13.
Upon invasion of red blood cells (RBCs), the Apicomplexa parasite Babesia divergens remains within the RBC for several hours and reproduces asexually, resulting in infective free merozoites that egress and destroy the host cell. Free merozoites rapidly seek and invade new uninfected RBCs. This repetitive cycle allows B. divergens to build a complex population of intraerythrocytic and extracellular stages in the bloodstream of humans and cattle, thus causing babesiosis. To compare biological aspects between B. divergens stages, including the different nature of their metabolism, could be key to our understanding of pathogenesis. Thus, we are currently assessing differences in the B. divergens metabolism of intra- and extracellular (free merozoites) life stages by the use of an integrative approach combining functional genomic, transcriptomic, differential expression, and metabolomic data acquired from sequencing and various analytical platforms. To our knowledge, this is the first effort to describe, in detail, the experimental procedures and integration of different omics to explore the regulation of the metabolism, invasion and proliferation mechanisms of B. divergens. This integrative approach can be used as a reference to study other Apicomplexa parasites.
当红细胞（RBC）受到入侵时，巴贝虫属寄生虫贝氏巴贝斯虫会在 RBC 内停留数小时并进行无性繁殖，产生感染性的游离裂殖子，这些裂殖子逸出并破坏宿主细胞。游离裂殖子迅速寻找并入侵新的未感染的 RBC。这种重复循环使 B. divergens 能够在人类和牛的血液中建立一个复杂的红细胞内和细胞外阶段的种群，从而导致巴贝斯虫病。比较 B. divergens 阶段的生物学方面，包括它们代谢的不同性质，可能是我们理解发病机制的关键。因此，我们目前正在通过整合功能基因组学、转录组学、差异表达和代谢组学数据的综合方法，来评估细胞内和细胞外（游离裂殖子）生活阶段的 B. divergens 代谢的差异。据我们所知，这是首次详细描述描述实验程序和不同组学的整合，以探索 B. divergens 代谢、入侵和增殖机制的调控。这种综合方法可以用作研究其他顶复门寄生虫的参考。
