Brodzki Piotr, Marczuk Jan, Lisiecka Urszula, Szczubiał Marek, Brodzki Adam, Gorzkoś Hubert, Kulpa Katarzyna
Department and Clinic of Animal Reproduction, Faculty of Veterinary Medicine, University of Life Science in Lublin, Lublin, Poland.
Department and Clinic of Animal Internal Medicine, Faculty of Veterinary Medicine, University of Life Science in Lublin, Lublin, Poland.
Vet World. 2021 Jun;14(6):1572-1578. doi: 10.14202/vetworld.2021.1572-1578. Epub 2021 Jun 18.
Ketosis is a common disease occurring during the first stage of lactation in highly productive dairy cows. The aim of the present study was the comparative assessment of selected pro-inflammatory cytokines (including tumor necrosis factor-α [TNF-α] and interleukin 6 [IL-6]), anti-inflammatory cytokines (including IL-10), and acute-phase proteins (APPs) (including haptoglobin [Hp] and serum amyloid A [SAA]), in the sera of cows with subclinical ketosis (SCK), in those with clinical ketosis (CK), and in healthy cows.
Thirty dairy cows of Holstein-Friesian breed were investigated. The cows were divided into three groups depending on the serum β-hydroxybutyric acid (βHBA) level. The control, SCK, and CK groups included healthy cows, cows with SCK, and cows with CK, respectively. BHBA concentration in blood serum was determined using colorimetric method. The blood serum was used for proper tests. Cytokine (TNF-α, IL-6, and IL-10) and APPs (SAA and Hp) concentrations in the investigated samples were determined by enzyme-linked immunosorbent assay method.
The SCK group had significantly higher TNF-α, IL-6; IL-10, and SAA values than had the CK group (p<0.01). The SCK group had a lower Hp concentration than had the CK group (p<0.05).
This study showed that the inflammation intensity is higher in the initial phase of the disease and decreases during the advancement, probably due to active anti-inflammatory mechanisms (an increase of IL-10 concentration), which protect animal organism from self-destruction. On the basis of our study, it can be assumed that ketosis development in dairy cows was preceded by the systemic inflammation that may influence the progress of this disease.
酮病是高产奶牛泌乳初期常见的疾病。本研究的目的是对亚临床酮病（SCK）奶牛、临床酮病（CK）奶牛和健康奶牛血清中选定的促炎细胞因子（包括肿瘤坏死因子-α [TNF-α] 和白细胞介素6 [IL-6]）、抗炎细胞因子（包括IL-10）和急性期蛋白（APPs）（包括触珠蛋白 [Hp] 和血清淀粉样蛋白A [SAA]）进行比较评估。
对30头荷斯坦-弗里生品种的奶牛进行了研究。根据血清β-羟基丁酸（βHBA）水平将奶牛分为三组。对照组、SCK组和CK组分别包括健康奶牛、患有SCK的奶牛和患有CK的奶牛。采用比色法测定血清中BHBA浓度。采集血清进行相关检测。采用酶联免疫吸附测定法测定所研究样本中细胞因子（TNF-α、IL-6和IL-10）和APPs（SAA和Hp）的浓度。
SCK组的TNF-α、IL-6、IL-10和SAA值显著高于CK组（p<0.01）。SCK组的Hp浓度低于CK组（p<0.05）。
本研究表明，疾病初期的炎症强度较高，在疾病进展过程中降低，这可能是由于活跃的抗炎机制（IL-10浓度升高），从而保护动物机体免于自我破坏。根据我们的研究，可以假设奶牛酮病的发生之前存在全身性炎症，这可能会影响该疾病的进展。
