Brodzki P, Kostro K, Brodzki A, Wawron W, Marczuk J, Kurek Ł
Department and Clinic of Reproduction, University of Life Sciences in Lublin, Lublin, Poland.
Department of Epizootiology and Clinic of Infectious Diseases, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Lublin, Poland.
Theriogenology. 2015 Jul 1;84(1):11-8. doi: 10.1016/j.theriogenology.2015.02.006. Epub 2015 Feb 12.
The aim of the study was to evaluate the level of proinflammatory cytokines (tumor necrosis factor-α [TNF-α], interleukin-6 [IL-6]), anti-inflammatory cytokine (interleukin-10 [IL-10]), and acute-phase proteins (haptoglobin [Hp] and serum amyloid A [SAA]) in serum and uterine washings in cows that developed endometritis during the early postpartum period. The study was carried out on 40 cows. The experimental group consisted of 20 cows with subclinical endometritis and the control group of 20 cows without endometritis. Analyses in both groups of cows were carried out at 5, 22, and 40 days postpartum (DPP). Experimental material consisted of the blood serum and uterine washings. The levels of the following cytokines: TNF-α, IL-6, IL-10 and acute-phase proteins: Hp and SAA were determined using ELISA. Our study reported that the levels of TNF-α, IL-6, IL-10, Hp, and SAA at 22 DPP were higher in cows with subclinical endometritis (P < 0.001). The levels of TNF-α (P = 0.01), IL-6 and IL-10 (P = 0.001), and Hp (P < 0.001) at 40 DPP were higher in cows with subclinical endometritis compared to healthy cows. The level of IL-10 in uterine washings at 5 DPP was higher (P = 0.001), whereas of SAA was lower (P = 0.01) in cows with subclinical endometritis. At 22 DPP, the levels of IL-6, IL-10, and Hp were higher (P < 0.001) in cows with endometritis. At 40 DPP, the level of TNF-α was lower, whereas these of IL-10 and Hp were elevated (P < 0.001) in cows with endometritis compared to healthy cows. The results indicate that the evaluation of the levels of cytokines and Hp in serum, but primarily in uterine washings, can be an important diagnostic indicator in cows that developed subclinical endometritis. High levels of IL-10 in cows with subclinical endometritis may contribute to the weakening of local resistance mechanisms of the uterus and lead to the persistence of the inflammation in the postpartum period. The present study also shows that the simultaneous examination of selected parameters of antagonistic interactions allows for better assessment of the current state of local immunity in the uterus.
本研究的目的是评估产后早期发生子宫内膜炎的奶牛血清和子宫冲洗液中促炎细胞因子（肿瘤坏死因子-α [TNF-α]、白细胞介素-6 [IL-6]）、抗炎细胞因子（白细胞介素-10 [IL-10]）和急性期蛋白（触珠蛋白 [Hp] 和血清淀粉样蛋白A [SAA]）的水平。该研究对40头奶牛进行。实验组由20头患有亚临床子宫内膜炎的奶牛组成，对照组由20头无子宫内膜炎的奶牛组成。两组奶牛均在产后5天、22天和40天（DPP）进行分析。实验材料包括血清和子宫冲洗液。使用酶联免疫吸附测定法（ELISA）测定以下细胞因子：TNF-α、IL-6、IL-10以及急性期蛋白：Hp和SAA的水平。我们的研究报告称，患有亚临床子宫内膜炎的奶牛在产后22天TNF-α、IL-6、IL-10、Hp和SAA的水平较高（P < 0.001）。与健康奶牛相比，患有亚临床子宫内膜炎的奶牛在产后40天TNF-α（P = 0.01）、IL-6和IL-10（P = 0.001）以及Hp（P < 0.001）的水平较高。患有亚临床子宫内膜炎的奶牛在产后5天子宫冲洗液中IL-10的水平较高（P = 0.001），而SAA的水平较低（P = 0.01）。在产后22天，患有子宫内膜炎的奶牛IL-6、IL-10和Hp的水平较高（P < 0.001）。在产后40天，与健康奶牛相比，患有子宫内膜炎的奶牛TNF-α的水平较低，而IL-10和Hp的水平升高（P < 0.001）。结果表明，评估血清中细胞因子和Hp的水平，但主要是子宫冲洗液中的水平，可能是产后发生亚临床子宫内膜炎奶牛的一项重要诊断指标。患有亚临床子宫内膜炎的奶牛中高水平的IL-10可能导致子宫局部抵抗机制减弱，并导致产后炎症持续存在。本研究还表明，同时检测拮抗相互作用的选定参数有助于更好地评估子宫局部免疫的当前状态。
