Division of State Patrol, Bureau of Transportation Safety, Chemical Testing Section, Wisconsin Department of Transportation, 3502 Kinsman Blvd., Madison, WI 53704, USA.
Wisconsin State Laboratory of Hygiene, Forensic Toxicology Division, University of Wisconsin-Madison, 2601 Agricultural Dr., Madison, WI 53718, USA.
J Anal Toxicol. 2022 Jul 14;46(6):683-688. doi: 10.1093/jat/bkab086.
In this work, 114 volunteers were dosed with 80-proof liquor to produce peak blood-alcohol concentration (BAC) or breath-alcohol concentration (BrAC) of 0.040-0.080 g/100 mL blood or g/210 L breath. This was followed by a 30 minute deprivation period before simultaneous blood and breath samples were collected and the alcohol concentration quantified. BAC was determined by gas chromatography with flame ionization detection and BrAC by a dual-sensor Intox EC/IR II instrument. Paired Student t-tests showed that differences between paired blood- and breath-alcohol results differed significantly. Results from these two measurement methods are highly correlated and, on average, measured BAC was 11.3% greater than BrAC. There were 10 instances of BrAC being greater than the corresponding BAC, and the average difference between these two values was 0.0059 g/100 mL. Agreement plots of coupled BAC and BrAC revealed a mean bias of 0.00754 g/100 mL and 95% limits of agreement (LOA) at -0.00705 and 0.0221 g/100 mL. Once BrAC values were truncated to the hundredths place as required by Wisconsin state statute, only three participants had greater BrAC than corresponding BAC, with an average difference between these values of 0.008 g/100 mL. Agreement plots with truncated BrAC values gave a mean bias of 0.0120 g/100 mL and 95% LOA at -0.00344 and 0.0275 g/100 mL. Data showed that typically, blood samples had greater alcohol concentrations than corresponding breath values. Differences were exacerbated by Wisconsin's statutory requirement that reported breath alcohol measurements be truncated to the hundredths place, whereas blood has no corresponding mandate.
在这项工作中，114 名志愿者摄入 80 度标准酒，使血液中的酒精浓度（BAC）或呼气中的酒精浓度（BrAC）达到 0.040-0.080g/100ml 血液或 g/210L 呼气。在这之后，志愿者需要经历 30 分钟的断酒期，然后同时采集血液和呼气样本，并定量检测酒精浓度。BAC 通过气相色谱法和火焰离子化检测来确定，BrAC 通过双传感器 Intox EC/IR II 仪器来确定。配对学生 t 检验显示，配对血样和呼出气样结果之间的差异有统计学意义。这两种测量方法的结果高度相关，平均而言，测量的 BAC 比 BrAC 高 11.3%。有 10 例 BrAC 高于相应的 BAC，这两个值之间的平均差异为 0.0059g/100ml。同时检测 BAC 和 BrAC 的一致性图显示，平均偏差为 0.00754g/100ml，95%一致性界限（LOA）在-0.00705 和 0.0221g/100ml 之间。一旦 BrAC 值按照威斯康星州法规的要求截断到百分位，只有 3 名参与者的 BrAC 高于相应的 BAC，这两个值之间的平均差异为 0.008g/100ml。截断 BrAC 值后的一致性图显示，平均偏差为 0.0120g/100ml，95%LOA 在-0.00344 和 0.0275g/100ml 之间。数据表明，通常情况下，血液样本中的酒精浓度高于相应的呼气值。这种差异在威斯康星州法规要求报告的呼气酒精测量值截断到百分位时更为明显，而血液样本没有这种要求。
