College of Natural Resources and Environment, Northwest A&F University, Yangling, Shaanxi 712100, China.
College of Natural Resources and Environment, Northwest A&F University, Yangling, Shaanxi 712100, China; Shaanxi Engineering Research Center of Utilization of Agricultural Waste Resources, Northwest A&F University, Yangling, Shaanxi 712100, China.
Bioresour Technol. 2021 Oct;337:125469. doi: 10.1016/j.biortech.2021.125469. Epub 2021 Jun 27.
Diazotroph community contributes to the nitrogen mass and improves the agronomic quality of composting product, but their responses to microbial inoculation during composting are unclear. In this study, the lignocellulose-degrading consortium was inoculated at different levels (0%: CK (control) and 10%: T) to investigate their effects on the variations in the diazotroph community and functional gene during composting. In the later composting phase, the nifH gene copy number was 17.50-25.28% higher in T than CK. The nitrogenase abundance in CK and T were 0.042% and 0.046% in composting product, respectively. Network analysis indicated that inoculation affected the co-occurrence patterns of the diazotroph community and changed the keystone species composition. Partial least-squares path modeling showed that available carbon sources and the succession of the diazotroph community mainly determined the increased abundance of nifH gene. Microbial inoculation stimulated the diazotrophs activities, and was conducive to the nitrogen production in composting product.
固氮菌群落有助于氮素质量的提高，并改善堆肥产品的农艺质量，但它们在堆肥过程中对微生物接种的反应尚不清楚。本研究在不同水平（0%：CK（对照）和 10%：T）下接种木质纤维素降解菌联合体，以研究它们对堆肥过程中固氮菌群落和功能基因变化的影响。在堆肥的后期，T 中的 nifH 基因拷贝数比 CK 高 17.50-25.28%。CK 和 T 中的固氮酶丰度分别为堆肥产品中的 0.042%和 0.046%。网络分析表明，接种影响了固氮菌群落的共存模式，并改变了关键种的组成。偏最小二乘路径模型显示，可用碳源和固氮菌群落的演替主要决定了 nifH 基因丰度的增加。微生物接种刺激了固氮菌的活性，有利于堆肥产品中氮素的产生。
