College of Natural Resources and Environment, Northwest A&F University, Yangling, Shaanxi 712100, China; Shaanxi Province Institute of Microbiology, Xian, Shaanxi 710043, China.
College of Natural Resources and Environment, Northwest A&F University, Yangling, Shaanxi 712100, China.
Bioresour Technol. 2019 Nov;291:121876. doi: 10.1016/j.biortech.2019.121876. Epub 2019 Jul 24.
Composting is used widely for recycling spent mushroom substrate (SMS). This study investigated the effects of inoculating a lignocellulose-degrading consortium at two levels comprising 0% (control: CK) and 10% (T) on the fungal community and cellulose-degrading genes during SMS co-composting with swine manure. Lignocellulose degradation rate in T was 8.77-34.45% higher compared with CK. Inoculation affected the distribution of the fungal community, increased the community diversity, and inhibited pathogens. Network analysis showed that inoculation changed the co-occurrence patterns of the fungal communities and made the co-composting system more stable. The relative abundances of glycoside hydrolase genes GH3E (fungal GH3), GH6, and GH7 were 0.45, 0.09, and 0.39 logs higher in T, respectively, than CK. Partial least-squares path modeling suggested that the variations in cellulose-degrading genes were driven mainly by changes in the fungal community during co-composting. Therefore, the lignocellulose-degrading consortium accelerated the transformation of lignocellulose to facilitate safer composting.
堆肥广泛用于回收废弃的蘑菇基质（SMS）。本研究调查了在两个水平（0%（对照：CK）和 10%（T））接种木质纤维素降解菌联合体对 SMS 与猪粪共堆肥过程中真菌群落和纤维素降解基因的影响。与 CK 相比，T 中的木质纤维素降解率高 8.77-34.45%。接种影响了真菌群落的分布，增加了群落的多样性，并抑制了病原体。网络分析表明，接种改变了真菌群落的共现模式，使共堆肥系统更加稳定。与 CK 相比，T 中糖苷水解酶基因 GH3E（真菌 GH3）、GH6 和 GH7 的相对丰度分别高出 0.45、0.09 和 0.39 个对数。偏最小二乘路径模型表明，在共堆肥过程中，纤维素降解基因的变化主要是由真菌群落的变化驱动的。因此，木质纤维素降解菌联合体加速了木质纤维素的转化，有助于更安全的堆肥。
