Chemical Engineering Laboratory, Faculty of Sciences and Centre for Advanced Scientific Research (CICA), BIOENGIN group, University of La Coruña, Rúa da Fraga 10, La Coruña 15008, Spain.
Chemical Engineering Laboratory, Faculty of Sciences and Centre for Advanced Scientific Research (CICA), BIOENGIN group, University of La Coruña, Rúa da Fraga 10, La Coruña 15008, Spain.
Bioresour Technol. 2021 Oct;337:125485. doi: 10.1016/j.biortech.2021.125485. Epub 2021 Jul 3.
The influence of the carbon source on the metabolism and growth of Clostridium aceticum was investigated, supplying either CO or fructose as sole carbon source. The acid and solvent production patterns were determined under either autotrophic or heterotrophic conditions, elucidating the effect of pH on the substrate's bioconversion pattern. The highest maximum specific growth rate was observed with CO, under the organism's optimal growth conditions, reaching 0.052 h and an acetic acid concentration of 18 g·L. The production of 4.4 g·L ethanol was also possible, after medium acidification, during CO bioconversion. Conversely, formic acid inhibition was observed during fructose fermentation under optimal growth conditions. In the latter experiments, it was not possible to stimulate solvent production when growing C. aceticum on fructose, despite applying the same medium acidification strategy as with CO, showing the selective effect of the carbon source (autotrophic vs heterotrophic) on the metabolic pattern and solventogenesis.
研究了碳源对醋化醋杆菌代谢和生长的影响，分别以 CO 和果糖作为唯一碳源。在自养或异养条件下确定了酸和溶剂的生产模式，阐明了 pH 值对底物生物转化模式的影响。在最佳生长条件下，CO 的最大比生长速率最高，达到 0.052 h，乙酸浓度为 18 g·L。在 CO 生物转化过程中，培养基酸化后还可以生产 4.4 g·L 的乙醇。相反，在最佳生长条件下，果糖发酵时会观察到甲酸抑制。在后一组实验中，尽管采用了与 CO 相同的培养基酸化策略，但在以果糖为碳源培养醋化醋杆菌时，无法刺激溶剂的产生，这表明碳源（自养与异养）对代谢模式和溶剂生成具有选择性影响。
