College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, No. 30 South Puzhu Road, Nanjing 211816, People's Republic of China.
Department of Bioengineering and Imperial College Centre for Synthetic Biology, Imperial College London, London SW7 2AZ, UK.
Bioresour Technol. 2021 Oct;337:125484. doi: 10.1016/j.biortech.2021.125484. Epub 2021 Jul 3.
The production of chemicals and fuels from lignocellulosic biomass has great potential industrial applications due to its economic feasibility and environmental attractiveness. However, the utilized microorganisms must be able to use all the sugars present in lignocellulosic hydrolysates, especially xylose, the second most plentiful monosaccharide on earth. Yarrowia lipolytica is a good candidate for producing various valuable products from biomass, but this yeast is unable to catabolize xylose efficiently. The development of metabolic engineering facilitated the application of Y. lipolytica as a platform for the bioconversion of xylose into various value-added products. Here, we reviewed the research progress on natural xylose-utilization pathways and their reconstruction in Y. lipolytica. The progress and emerging trends in metabolic engineering of Y. lipolytica for producing chemicals and fuels are further introduced. Finally, challenges and future perspectives of using lignocellulosic hydrolysate as substrate for Y. lipolytica are discussed.
木质纤维素生物质转化为化学品和燃料具有很大的工业应用潜力，因为它具有经济可行性和环境吸引力。然而，所用的微生物必须能够利用木质纤维素水解物中所有的糖，特别是木糖，木糖是地球上第二丰富的单糖。解脂耶氏酵母是一种从生物质生产各种有价值产品的良好候选者，但这种酵母不能有效地代谢木糖。代谢工程的发展促进了将解脂耶氏酵母作为将木糖生物转化为各种增值产品的平台的应用。在这里，我们综述了天然木糖利用途径及其在解脂耶氏酵母中的重建的研究进展。进一步介绍了解脂耶氏酵母用于生产化学品和燃料的代谢工程的进展和新兴趋势。最后，讨论了使用木质纤维素水解物作为解脂耶氏酵母的底物的挑战和未来展望。
