Hauswirth J W
Biochemistry Research Division, Sinai Hospital of Baltimore, Inc., MD 21215.
Nutr Cancer. 1987;10(4):215-20. doi: 10.1080/01635588709513959.
After being maintained on a vitamin A-deficient or complete diet for a period of five weeks, male Sprague-Dawley rats were subjected to a two-thirds partial hepatectomy (PH) or sham operation. The vitamin A content of the liver of vitamin A-deficient, PH rats was below the limit of detection (less than 1 microgram/g liver). Rats fed the control diet and subjected to PH had hepatic levels of vitamin A that were 37% and 49% lower 48 and 72 hours after surgery, respectively, when compared with sham-operated controls. Hepatic microsomal cytochrome P-450 levels were significantly reduced in PH rats fed the complete diet 48 hours after PH and in PH rats fed either the deficient or complete diet 72 hours after. Vitamin A deficiency alone significantly reduced cytochrome P-450 levels. A combination of vitamin A deficiency and PH had the most dramatic effect on cytochrome P-450 and aminopyrine N-demethylase, which reduced the activity to approximately 50% of the activities found in the sham-operated control group. PH resulted in the greatest reduction in the rate of disappearance of benzo[a]pyrene in the presence of liver microsomes prepared from vitamin A-deficient rats.
雄性斯普拉格-道利大鼠在维生素A缺乏或完全饮食条件下饲养五周后，接受了三分之二部分肝切除术（PH）或假手术。维生素A缺乏的PH大鼠肝脏中的维生素A含量低于检测限（低于1微克/克肝脏）。与假手术对照组相比，喂食对照饮食并接受PH的大鼠在术后48小时和72小时肝脏中的维生素A水平分别降低了37%和49%。在PH术后48小时，喂食完全饮食的PH大鼠以及在术后72小时，喂食缺乏或完全饮食的PH大鼠肝脏微粒体细胞色素P-450水平均显著降低。单独的维生素A缺乏会显著降低细胞色素P-450水平。维生素A缺乏与PH的组合对细胞色素P-450和氨基比林N-脱甲基酶的影响最为显著，其活性降低至假手术对照组的约50%。在由维生素A缺乏的大鼠制备的肝脏微粒体存在的情况下，PH导致苯并[a]芘消失速率的最大降低。
